Department of Biological Sciences, Technology Center for Networks and Pathways, Molecular Biosensor and Imaging Center, Carnegie Mellon University, Pittsburgh, Pennsylvania 15213, USA.
Cytometry A. 2010 Aug;77(8):776-82. doi: 10.1002/cyto.a.20925.
This study explores the general utility of a new class of biosensor that allows one to selectively visualize molecules of a chosen membrane protein that are at the cell surface. These biosensors make use of recently described bipartite fluoromodules comprised of a fluorogen-activating protein (FAP) and a small molecule (fluorogen) whose fluorescence increases dramatically when noncovalently bound by the FAP (Szent-Gyorgyi et al., Nat Biotechnol 2010;00:000-000).
本研究探讨了一类新型生物传感器的普遍适用性,该传感器可以选择性地可视化位于细胞表面的选定膜蛋白的分子。这些生物传感器利用最近描述的由荧光团激活蛋白 (FAP) 和小分子 (荧光团) 组成的双部分荧光团模块,当非共价结合 FAP 时,其荧光会显著增加 (Szent-Gyorgyi 等人,Nat Biotechnol 2010;00:000-000)。