School of Medicine, University of Pittsburgh, Pittsburgh, Pennsylvania, USA.
The Department of Biological Sciences, Carnegie Mellon, Pittsburgh, Pennsylvania, USA.
Traffic. 2020 Apr;21(4):333-348. doi: 10.1111/tra.12722. Epub 2020 Mar 1.
Throughout the past decade the use of fluorogen activating proteins (FAPs) has expanded with several unique reporter dyes that support a variety of methods to specifically quantify protein trafficking events. The platform's capabilities have been demonstrated in several systems and shared for widespread use. This review will highlight the current FAP labeling techniques for protein traffic measurements and focus on the use of the different designed fluorogenic dyes for selective and specific labeling applications.
在过去的十年中,荧光蛋白激活蛋白(FAPs)的应用得到了扩展,出现了几种独特的报告染料,支持多种方法来专门定量蛋白质运输事件。该平台的功能已在多个系统中得到验证,并被广泛共享。本综述将重点介绍当前用于蛋白质流量测量的 FAP 标记技术,并关注不同设计的荧光染料在选择性和特异性标记应用中的使用。