Bleyl Jörg U, Heller Axel R, Fehrenbach Antonia, Heintz Manuel, Fehrenbach Heinz, Klenz Gesa, Gama de Abreu Marcelo, Hübler Matthias, Spieth Peter M, Koch Thea
Clinic for Anaesthesiology and Intensive Care Therapy, University Hospital Carl Gustav Carus, Technical University of Dresden, Dresden, Germany.
Exp Lung Res. 2010 Aug;36(6):342-51. doi: 10.3109/01902141003653320.
The authors investigated the protective effects and dose dependency of perfluorohexane (PFH) vapor on leukocyte-mediated lung injury in isolated, perfused, and ventilated rabbit lungs. Lungs received either 18 vol.% (n = 7), 9 vol.% (n = 7), or 4.5 vol.% (n = 7) PFH. Fifteen minutes after beginning of PFH application, lung injury was induced with formyl-Met-Leu-Phe (fMLP). Control lungs (n = 7) received fMLP only. In addition 5 lungs (PFH-sham) remained uninjured receiving 18 vol.% PFH only. Pulmonary artery pressure (mPAP), peak inspiratory pressure (P(max)), and lung weight were monitored for 90 minutes. Perfusate samples were taken at regular intervals for analysis and representative lungs were fixed for histological analysis. In the control, fMLP application led to a significant increase of mPAP, P(max), lung weight, and lipid mediators. Pretreatment with PFH attenuated the rise in these parameters. This was accompanied by preservation of the structural integrity of the alveolar architecture and air-blood barrier. In uninjured lungs, mPAP, P(max), lung weight, and lipid mediator formation remained uneffected in the presence of PFH. The authors concluded that pretreatment with PFH vapor leads to an attenuation of leukocyte-mediated lung injury. Vaporization of perfluorocarbons (PFCs) offers new therapeutic options, making use of their protective and anti-inflammatory properties in prophylaxis or in early treatment of acute lung injury.
作者研究了全氟己烷（PFH）蒸汽对离体、灌注和通气兔肺中白细胞介导的肺损伤的保护作用及其剂量依赖性。肺分别接受18体积%（n = 7）、9体积%（n = 7）或4.5体积%（n = 7）的PFH。在开始应用PFH 15分钟后，用甲酰甲硫氨酰亮氨酰苯丙氨酸（fMLP）诱导肺损伤。对照肺（n = 7）仅接受fMLP。此外，5只肺（PFH假手术组）仅接受18体积%的PFH，未受损伤。监测肺动脉压（mPAP）、吸气峰压（P(max)）和肺重量90分钟。定期采集灌注液样本进行分析，并将代表性的肺固定用于组织学分析。在对照组中，应用fMLP导致mPAP、P(max)、肺重量和脂质介质显著增加。PFH预处理减弱了这些参数的升高。这伴随着肺泡结构和气血屏障结构完整性的保留。在未受损伤的肺中，PFH存在时mPAP、P(max)、肺重量和脂质介质的形成未受影响。作者得出结论，PFH蒸汽预处理可减轻白细胞介导的肺损伤。全氟化碳（PFCs）的汽化提供了新的治疗选择，利用其在预防或急性肺损伤早期治疗中的保护和抗炎特性。
