Hiraba Hisao, Sato Takako, Nakayama Enri, Yamaoka Masaru, Inoue Motoharu, Sato Mitsuyasu, Iida Takatoshi, Wada Satoko, Ueda Koichiro
Department of Dysphasia Rehabilitation, Nihon University, School of Dentistry, Chiyoda-ku, Tokyo, Japan.
Somatosens Mot Res. 2010;27(3):93-9. doi: 10.3109/08990220.2010.499754.
We studied changes in orofacial behavior and the arrangement of bilateral hypoglossal nuclei after the neurectomy of the medial branch of the unilateral hypoglossal nerve in cats. After recovery from surgery in a head holder, the animals were acclimated to take and chew fish paste (1.8 g) from a spoon and lick milk from a wetted paintbrush. Next we performed a neurectomy in the unilateral hypoglossal nerve after training. We firstly recorded behavior during the taking of fish paste and licking of milk, and then performed a neurectomy in the unilateral hypoglossal nerve. After nerve cutting, the cats' tongue deviated toward the cut side when they licked food, and bilateral activities of EMGs in the genioglossus muscles became stable in about 1 month. After that, we injected two kinds of fluorescent dye (10% Evans blue, EB, and 3% Fast blue, FB) into the bilateral genioglossus muscles using syringes (0.15 ml in each), respectively. Although each injection of FB and EB into the bilateral genioglossus muscles in normal cats revealed cells positively stained with each dye in the hypoglossal nuclei of each injection site, in cats 1 month after nerve cutting, fluorescent dye was only observed in positive cells in the hypoglossal nucleus of the intact side and the dye injected into the neurectomy side showed a mixture into positive cells of the intact side. The findings suggest that muscles in the neurectomy side may be compensated by regeneration of the peripheral nerves on the intact side.
我们研究了猫单侧舌下神经内侧支神经切除术后口面部行为的变化以及双侧舌下神经核的排列情况。在头固定器中从手术恢复后，使动物适应从勺子中取食并咀嚼鱼酱（1.8克）以及从湿润的画笔上舔舐牛奶。接下来，在训练后对单侧舌下神经进行神经切除术。我们首先记录取食鱼酱和舔舐牛奶时的行为，然后对单侧舌下神经进行神经切除术。切断神经后，猫舔食食物时舌头偏向切断侧，颏舌肌肌电图的双侧活动在约1个月后变得稳定。之后，我们分别使用注射器（每侧0.15毫升）将两种荧光染料（10%伊文思蓝，EB，和3%固蓝，FB）注入双侧颏舌肌。尽管在正常猫中将FB和EB分别注入双侧颏舌肌时，在每个注射部位的舌下神经核中均显示有被每种染料阳性染色的细胞，但在神经切断1个月后的猫中，仅在完整侧的舌下神经核中的阳性细胞中观察到荧光染料，而注入神经切除侧的染料则混入了完整侧的阳性细胞中。这些发现表明，神经切除侧的肌肉可能通过完整侧外周神经的再生得到补偿。
