Centro de Investigação em Química (CIQ-UP), Grupo de Ciências Biológicas e Bioanalíticas, Faculdade de Farmácia da Universidade de Coimbra, Polo das Ciências da Saúde, 3000-548 Coimbra, Portugal.
J Photochem Photobiol B. 2010 Oct 5;101(1):1-8. doi: 10.1016/j.jphotobiol.2010.06.015. Epub 2010 Jul 3.
Firefly luciferase (Luc) is the most studied of the luciferase enzymes and the mechanism and kinetics of the reactions catalyzed by this enzyme have been relatively well characterized. Luc catalyzes the bioluminescent reaction involving firefly luciferin (D-LH(2)), adenosine triphosphate (ATP), magnesium ion and molecular oxygen with the formation of an electronically excited species (oxyluciferin), inorganic pyrophosphate (PPi), carbon dioxide and adenosine monophosphate (AMP). Luc also catalyzes other non-luminescent reactions, which can interfere with the light production mechanism. Following electronic relaxation, the excited oxyluciferin emits radiation in the visible region of the electromagnetic spectrum (550-570 nm). Among the various possible compounds, several classes of inhibitory substances interfere with the activity of this enzyme: here, we consider substrate-related compounds, intermediates or products of the Luc catalyzed reactions, in addition to anesthetics and, fatty acids. This review summarizes the main inhibitors of Luc and the corresponding inhibition kinetic parameters.
萤火虫荧光素酶（Luc）是研究最广泛的荧光素酶之一，其催化反应的机制和动力学已经得到了相对较好的描述。Luc 催化涉及萤火虫荧光素（D-LH(2)）、三磷酸腺苷（ATP）、镁离子和分子氧的生物发光反应，形成一个电子激发态物种（氧化荧光素）、无机焦磷酸（PPi）、二氧化碳和一磷酸腺苷（AMP）。Luc 还催化其他非发光反应，这些反应可能会干扰发光机制。电子弛豫后，激发态氧化荧光素在电磁光谱的可见区域（550-570nm）发射辐射。在各种可能的化合物中，有几类抑制物质会干扰该酶的活性：在这里，我们除了考虑麻醉剂和脂肪酸外，还考虑与 Luc 催化反应的底物相关的化合物、中间产物或产物。本综述总结了 Luc 的主要抑制剂及其相应的抑制动力学参数。
