The Elie Metchnikoff Department, University of Messina, Messina, Italy.
J Proteomics. 2010 Nov 10;73(12):2365-9. doi: 10.1016/j.jprot.2010.07.009. Epub 2010 Jul 23.
Streptococcus suis serotype 2 is a major Gram-positive swine pathogen, causing also zoonoses. We describe here the immunoprotective activity in an in vivo animal model of a serotype-2 cell wall protein, designated Sat, which was identified by a previously validated proteomics approach consisting of the protease digestion of live bacteria and the selective recovery of exposed domains, followed by LC/MS/MS analysis. Increased survival rate (80%) and decreased bacterial burden were observed in mice immunized with a recombinant Sat fragment, suggesting that this protein is a potential vaccine candidate against serotype-2 infection.
猪链球菌 2 型是一种主要的革兰氏阳性猪病原体，也会引起人畜共患病。我们在此描述了一种通过先前验证的蛋白质组学方法鉴定的 2 型细胞壁蛋白 Sat 在体内动物模型中的免疫保护活性。该方法包括对活菌进行蛋白酶消化，选择性回收暴露的结构域，然后进行 LC/MS/MS 分析。用重组 Sat 片段免疫的小鼠的存活率（80%）增加，细菌负荷减少，表明该蛋白是针对 2 型感染的潜在疫苗候选物。
