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从HeLa细胞核提取物中亲和纯化转录因子IIA。

Affinity purification of transcription factor IIA from HeLa cell nuclear extracts.

作者信息

Usuda Y, Kubota A, Berk A J, Handa H

机构信息

Department of Bacteriology, University of Tokyo Faculty of Medicine, Japan.

出版信息

EMBO J. 1991 Aug;10(8):2305-10. doi: 10.1002/j.1460-2075.1991.tb07767.x.

DOI:10.1002/j.1460-2075.1991.tb07767.x
PMID:2065666
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC452922/
Abstract

One of the general transcription factors, TFIIA, was purified to homogeneity from HeLa cell nuclear extracts by yeast TFIID affinity chromatography. Human TFIIA had a molecular weight of approximately 38 kd. It was able to associate with the complex formed by yeast TFIID and the TATA elements of the adenovirus E4 and ML promoters, and the HSP70 promoter. The association extended the protected region on each TATA element by yeast TFIID from DNase I digestion. Affinity-purified TFIIA was also able to stimulate transcription from the E4 and ML promoters in in vitro reconstituted systems.

摘要

通用转录因子之一TFIIA，通过酵母TFIID亲和层析从HeLa细胞核提取物中纯化至同质。人TFIIA的分子量约为38kd。它能够与酵母TFIID与腺病毒E4和ML启动子以及HSP70启动子的TATA元件形成的复合物结合。这种结合扩展了酵母TFIID对每个TATA元件免受DNase I消化的保护区域。亲和纯化的TFIIA在体外重组系统中也能够刺激E4和ML启动子的转录。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f73/452922/954917582822/emboj00106-0336-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f73/452922/46b6fd24d4ad/emboj00106-0334-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f73/452922/09a343ca82a5/emboj00106-0334-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f73/452922/b41109dcce38/emboj00106-0335-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f73/452922/e99bdcc3ca08/emboj00106-0335-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f73/452922/df0fc6076336/emboj00106-0335-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f73/452922/26c9478a7f47/emboj00106-0336-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f73/452922/954917582822/emboj00106-0336-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f73/452922/46b6fd24d4ad/emboj00106-0334-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f73/452922/09a343ca82a5/emboj00106-0334-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f73/452922/b41109dcce38/emboj00106-0335-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f73/452922/e99bdcc3ca08/emboj00106-0335-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f73/452922/df0fc6076336/emboj00106-0335-c.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f73/452922/26c9478a7f47/emboj00106-0336-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/7f73/452922/954917582822/emboj00106-0336-b.jpg

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本文引用的文献

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Separation and characterization of factors mediating accurate transcription by RNA polymerase II.介导RNA聚合酶II精确转录的因子的分离与特性分析。
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Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.从分离的哺乳动物细胞核的可溶性提取物中，RNA聚合酶II进行准确的转录起始。
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Formation of stable preinitiation complexes between eukaryotic class B transcription factors and promoter sequences.
小麦中转录激活的共激活因子和TATA结合蛋白相关因子
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Transcription factor IIA stimulates the expression of classical polIII-genes.转录因子IIA刺激经典的RNA聚合酶III基因的表达。
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Distinct TFIID complexes mediate the effect of different transcriptional activators.不同的TFIID复合物介导不同转录激活因子的作用。
EMBO J. 1993 Feb;12(2):489-99. doi: 10.1002/j.1460-2075.1993.tb05681.x.
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A TATA sequence-dependent transcriptional repressor activity associated with mammalian transcription factor IIA.一种与哺乳动物转录因子IIA相关的依赖TATA序列的转录抑制活性。
EMBO J. 1994 Jan 15;13(2):435-45. doi: 10.1002/j.1460-2075.1994.tb06278.x.
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Mediators of activation of fushi tarazu gene transcription by BmFTZ-F1.BmFTZ-F1激活腹节基因转录的介质
Mol Cell Biol. 1994 May;14(5):3013-21. doi: 10.1128/mcb.14.5.3013-3021.1994.
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Transcription factors required for the expression of Xenopus laevis selenocysteine tRNA in vitro.非洲爪蟾硒代半胱氨酸tRNA体外表达所需的转录因子。
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Nature. 1983 Feb 24;301(5902):680-6. doi: 10.1038/301680a0.
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Interactions between RNA polymerase II, factors, and template leading to accurate transcription.RNA聚合酶II、因子与模板之间的相互作用导致精确转录。
J Biol Chem. 1984 Feb 25;259(4):2509-16.
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Is actin a transcription initiation factor for RNA polymerase B?肌动蛋白是RNA聚合酶B的转录起始因子吗？
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Stimulation of in vitro transcription by the upstream element of the adenovirus-2 major late promoter involves a specific factor.腺病毒2型主要晚期启动子上游元件对体外转录的刺激涉及一种特异性因子。
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Interaction of a gene-specific transcription factor with the adenovirus major late promoter upstream of the TATA box region.一种基因特异性转录因子与腺病毒主要晚期启动子在TATA框区域上游的相互作用。
Cell. 1985 Nov;43(1):165-75. doi: 10.1016/0092-8674(85)90021-2.
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Purification and characterization of a specific RNA polymerase II transcription factor.一种特异性RNA聚合酶II转录因子的纯化与特性分析
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Factors involved in specific transcription by human RNA polymerase II: analysis by a rapid and quantitative in vitro assay.人RNA聚合酶II特异性转录所涉及的因素：通过快速定量体外分析进行研究
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Isolation of three proteins that bind to mammalian RNA polymerase II.三种与哺乳动物RNA聚合酶II结合的蛋白质的分离。
J Biol Chem. 1985 Aug 25;260(18):10353-60.