从分离的哺乳动物细胞核的可溶性提取物中，RNA聚合酶II进行准确的转录起始。
Accurate transcription initiation by RNA polymerase II in a soluble extract from isolated mammalian nuclei.
作者信息
Dignam J D, Lebovitz R M, Roeder R G
出版信息
Nucleic Acids Res. 1983 Mar 11;11(5):1475-89. doi: 10.1093/nar/11.5.1475.
We have developed a procedure for preparing extracts from nuclei of human tissue culture cells that directs accurate transcription initiation in vitro from class II promoters. Conditions of extraction and assay have been optimized for maximum activity using the major late promoter of adenovirus 2. The extract also directs accurate transcription initiation from other adenovirus promoters and cellular promoters. The extract also directs accurate transcription initiation from class III promoters (tRNA and Ad 2 VA).
我们已经开发出一种从人组织培养细胞核中制备提取物的方法，该提取物可在体外指导从II类启动子进行精确的转录起始。使用腺病毒2的主要晚期启动子，对提取和检测条件进行了优化以获得最大活性。该提取物还可指导从其他腺病毒启动子和细胞启动子进行精确的转录起始。该提取物还可指导从III类启动子（tRNA和腺病毒2 VA）进行精确的转录起始。
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