A 重复序列将 ASF/SF2 依赖性 Xist RNA 加工与 X 染色体失活过程中的随机选择联系起来。
The A-repeat links ASF/SF2-dependent Xist RNA processing with random choice during X inactivation.
机构信息
Department of Biochemistry and Biophysics, University of California, San Francisco, California, USA.
出版信息
Nat Struct Mol Biol. 2010 Aug;17(8):948-54. doi: 10.1038/nsmb.1877. Epub 2010 Jul 25.
Abstract
One X chromosome, selected at random, is silenced in each female mammalian cell. Xist encodes a noncoding RNA that influences the probability that the cis-linked X chromosome will be silenced. We found that the A-repeat, a highly conserved element within Xist, is required for the accumulation of spliced Xist RNA. In addition, the A-repeat is necessary for X-inactivation to occur randomly. In combination, our data suggest that normal Xist RNA processing is important in the regulation of random X-inactivation. We propose that modulation of Xist RNA processing may be part of the stochastic process that determines which X chromosome will be inactivated.
摘要
在每个雌性哺乳动物细胞中，随机选择一条 X 染色体使其沉默。Xist 编码一种非编码 RNA，影响顺式连接的 X 染色体沉默的概率。我们发现，Xist 内高度保守的 A 重复序列对于拼接的 Xist RNA 的积累是必需的。此外，A 重复序列对于随机的 X 失活也是必需的。综合起来，我们的数据表明，正常的 Xist RNA 加工对于随机 X 失活的调节是很重要的。我们提出，Xist RNA 加工的调节可能是决定哪个 X 染色体失活的随机过程的一部分。
相似文献
1
The A-repeat links ASF/SF2-dependent Xist RNA processing with random choice during X inactivation.A 重复序列将 ASF/SF2 依赖性 Xist RNA 加工与 X 染色体失活过程中的随机选择联系起来。
Nat Struct Mol Biol. 2010 Aug;17(8):948-54. doi: 10.1038/nsmb.1877. Epub 2010 Jul 25.
2
A proximal conserved repeat in the Xist gene is essential as a genomic element for X-inactivation in mouse.Xist基因中的近端保守重复序列作为一种基因组元件，对小鼠的X染色体失活至关重要。
Development. 2009 Jan;136(1):139-46. doi: 10.1242/dev.026427. Epub 2008 Nov 26.
3
Spen links RNA-mediated endogenous retrovirus silencing and X chromosome inactivation.Spen 连接 RNA 介导的内源性逆转录病毒沉默和 X 染色体失活。
Elife. 2020 May 7;9:e54508. doi: 10.7554/eLife.54508.
4
A novel role for Xist RNA in the formation of a repressive nuclear compartment into which genes are recruited when silenced.Xist RNA在形成抑制性核区室中的新作用，基因沉默时会被招募到该核区室中。
Genes Dev. 2006 Aug 15;20(16):2223-37. doi: 10.1101/gad.380906.
5
Disruption of a conserved region of Xist exon 1 impairs Xist RNA localisation and X-linked gene silencing during random and imprinted X chromosome inactivation.干扰 Xist 外显子 1 保守区域会影响 Xist RNA 的定位，并在随机和印迹 X 染色体失活过程中导致 X 连锁基因沉默。
Development. 2011 Apr;138(8):1541-50. doi: 10.1242/dev.056812. Epub 2011 Mar 9.
6
2-D structure of the A region of Xist RNA and its implication for PRC2 association.Xist RNA A 区域的 2-D 结构及其对 PRC2 结合的影响。
PLoS Biol. 2010 Jan;8(1):e1000276. doi: 10.1371/journal.pbio.1000276. Epub 2010 Jan 5.
7
Elements at the 5' end of Xist harbor SPEN-independent transcriptional antiterminator activity.Xist 5' 端的元件具有 SPEN 非依赖性转录抗终止活性。
Nucleic Acids Res. 2020 Oct 9;48(18):10500-10517. doi: 10.1093/nar/gkaa789.
8
XIST RNA and architecture of the inactive X chromosome: implications for the repeat genome.XIST RNA与失活X染色体的结构：对重复基因组的影响
Cold Spring Harb Symp Quant Biol. 2010;75:345-56. doi: 10.1101/sqb.2010.75.030. Epub 2011 Mar 29.
9
Eutherian mammals use diverse strategies to initiate X-chromosome inactivation during development.真兽类哺乳动物在发育过程中使用多种策略来启动 X 染色体失活。
Nature. 2011 Apr 21;472(7343):370-4. doi: 10.1038/nature09872. Epub 2011 Apr 6.
10
X-inactivation: quantitative predictions of protein interactions in the Xist network.X 染色体失活：Xist 网络中蛋白质相互作用的定量预测。
Nucleic Acids Res. 2013 Jan 7;41(1):e31. doi: 10.1093/nar/gks968. Epub 2012 Oct 22.
引用本文的文献
1
A streamlined workflow for long-read DNA methylation analysis with NanoMethViz and Bioconductor.使用NanoMethViz和Bioconductor进行长读长DNA甲基化分析的简化工作流程。
F1000Res. 2025 Feb 24;13:1243. doi: 10.12688/f1000research.155204.2. eCollection 2024.
2
Measuring X-Chromosome inactivation skew for X-linked diseases with adaptive nanopore sequencing.利用自适应纳米孔测序技术测量 X 连锁疾病的 X 染色体失活偏倚。
Genome Res. 2024 Nov 20;34(11):1954-1965. doi: 10.1101/gr.279396.124.
3
Unraveling the role of Xist in X chromosome inactivation: insights from rabbit model and deletion analysis of exons and repeat A.揭示 Xist 在 X 染色体失活中的作用：来自兔模型和外显子及重复 A 缺失分析的见解。
Cell Mol Life Sci. 2024 Mar 29;81(1):156. doi: 10.1007/s00018-024-05151-0.
4
Trisomy silencing by XIST: translational prospects and challenges.XIST介导的三体沉默：转化前景与挑战。
Hum Genet. 2024 Jul;143(7):843-855. doi: 10.1007/s00439-024-02651-8. Epub 2024 Mar 9.
5
Regulatory principles and mechanisms governing the onset of random X-chromosome inactivation.调控随机 X 染色体失活起始的原则和机制。
Curr Opin Genet Dev. 2023 Aug;81:102063. doi: 10.1016/j.gde.2023.102063. Epub 2023 Jun 23.
6
BAF complex-mediated chromatin relaxation is required for establishment of X chromosome inactivation.BAF 复合物介导的染色质弛豫对于建立 X 染色体失活是必需的。
Nat Commun. 2022 Mar 29;13(1):1658. doi: 10.1038/s41467-022-29333-1.
7
The control of polycomb repressive complexes by long noncoding RNAs.长非编码 RNA 对 polycomb 抑制复合物的调控。
Wiley Interdiscip Rev RNA. 2021 Nov;12(6):e1657. doi: 10.1002/wrna.1657. Epub 2021 Apr 16.
8
Independent domains for recruitment of PRC1 and PRC2 by human XIST.人类 XIST 通过独立结构域招募 PRC1 和 PRC2
PLoS Genet. 2021 Mar 22;17(3):e1009123. doi: 10.1371/journal.pgen.1009123. eCollection 2021 Mar.
9
Deletion of the XIST promoter from the human inactive X chromosome compromises polycomb heterochromatin maintenance.从人类失活 X 染色体中删除 XIST 启动子会影响多梳异染色质的维持。
Chromosoma. 2021 Sep;130(2-3):177-197. doi: 10.1007/s00412-021-00754-z. Epub 2021 Mar 21.
10
Novel lnc-HZ03 and miR-hz03 promote BPDE-induced human trophoblastic cell apoptosis and induce miscarriage by upregulating p53/SAT1 pathway.新型长链非编码RNA-HZ03和微小RNA-hz03通过上调p53/SAT1通路促进苯并[a]芘二醇环氧化物诱导的人滋养层细胞凋亡并导致流产。
Cell Biol Toxicol. 2021 Dec;37(6):951-970. doi: 10.1007/s10565-021-09583-3. Epub 2021 Feb 10.
本文引用的文献
1
Condensin complexes regulate mitotic progression and interphase chromatin structure in embryonic stem cells.凝聚素复合物调节胚胎干细胞有丝分裂进程和间期染色质结构。
J Cell Biol. 2010 Feb 22;188(4):491-503. doi: 10.1083/jcb.200908026.
2
2-D structure of the A region of Xist RNA and its implication for PRC2 association.Xist RNA A 区域的 2-D 结构及其对 PRC2 结合的影响。
PLoS Biol. 2010 Jan;8(1):e1000276. doi: 10.1371/journal.pbio.1000276. Epub 2010 Jan 5.
3
A proximal conserved repeat in the Xist gene is essential as a genomic element for X-inactivation in mouse.Xist基因中的近端保守重复序列作为一种基因组元件，对小鼠的X染色体失活至关重要。
Development. 2009 Jan;136(1):139-46. doi: 10.1242/dev.026427. Epub 2008 Nov 26.
4
Dicer regulates Xist promoter methylation in ES cells indirectly through transcriptional control of Dnmt3a.Dicer 通过转录调控 Dnmt3a 间接调控 ES 细胞中的 Xist 启动子甲基化。
Epigenetics Chromatin. 2008 Oct 27;1(1):2. doi: 10.1186/1756-8935-1-2.
5
Polycomb proteins targeted by a short repeat RNA to the mouse X chromosome.一种短重复RNA靶向小鼠X染色体的多梳蛋白。
Science. 2008 Oct 31;322(5902):750-6. doi: 10.1126/science.1163045.
6
An RNAi screen of chromatin proteins identifies Tip60-p400 as a regulator of embryonic stem cell identity.一项针对染色质蛋白的RNA干扰筛选鉴定出Tip60-p400作为胚胎干细胞特性的调节因子。
Cell. 2008 Jul 11;134(1):162-74. doi: 10.1016/j.cell.2008.05.031.
7
SmcHD1, containing a structural-maintenance-of-chromosomes hinge domain, has a critical role in X inactivation.含有染色体结构维持铰链结构域的SmcHD1在X染色体失活中起关键作用。
Nat Genet. 2008 May;40(5):663-9. doi: 10.1038/ng.142. Epub 2008 Apr 20.
8
X inactivation counting and choice is a stochastic process: evidence for involvement of an X-linked activator.X染色体失活计数与选择是一个随机过程：X连锁激活因子参与的证据
Cell. 2008 Feb 8;132(3):410-21. doi: 10.1016/j.cell.2007.12.036.
9
Antisense artifacts in transcriptome microarray experiments are resolved by actinomycin D.转录组微阵列实验中的反义假象可通过放线菌素D解决。
Nucleic Acids Res. 2007;35(19):e128. doi: 10.1093/nar/gkm683. Epub 2007 Sep 26.
10
X inactivation Xplained.X染色体失活解析
Curr Opin Genet Dev. 2007 Oct;17(5):387-93. doi: 10.1016/j.gde.2007.08.001. Epub 2007 Sep 14.
Zotero 插件