大鼠胰部分切除术会导致葡萄糖诱导的胰岛血流刺激受损。

Partial pancreatectomy in rats causes an impairment of the glucose-induced stimulation of pancreatic islet blood flow.

作者信息

Jansson L, Sandler S

机构信息

Department of Medical Cell Biology, Biomedical Center, Uppsala University, Sweden.

出版信息

Experientia. 1991 Jun 15;47(6):627-9. doi: 10.1007/BF01949892.

DOI:10.1007/BF01949892

PMID:2065764

Abstract

Adult rats were subjected to either a sham operation (S-rats) or a 60% partial pancreatectomy (P-rats). Both P- and S-rats were normoglycemic and normoinsulinemic after surgery. Four weeks later, the animals were injected i.v. with 1 ml of either 0.9% (w/v) saline or 30% (w/v) D-glucose, and after 5 min whole pancreatic blood flow (PBF) and islet blood flow (IBF) were measured, using a microsphere technique. In the saline-injected P-rats both PBF and IBF values were higher than in S-rats (p less than 0.001 for both values). Administration of glucose had no effects on PBF in either S- or P-rats when compared to saline-injected animals. IBF was, however, markedly increased (p less than 0.01) by glucose in S-rats in comparison with saline-injected S-rats, whilst no difference in IBF was observed between glucose- and saline-injected P-rats. The fraction of PBF diverted through the islets (fIBF) was approximately 10% in S-rats and 20% in P-rats. Glucose increased fIBF in S-rats, but had no effect in P-rats. In conclusion, in S-rats a glucose-stimulated insulin release is accompanied by an increase in IBF, but this is not observed in P-rats.

摘要

成年大鼠接受假手术（假手术组大鼠）或60%胰腺部分切除术（手术组大鼠）。术后手术组和假手术组大鼠血糖和胰岛素水平均正常。四周后，给动物静脉注射1 ml 0.9%（w/v）生理盐水或30%（w/v）D -葡萄糖，5分钟后，采用微球技术测量全胰腺血流量（PBF）和胰岛血流量（IBF）。在注射生理盐水的手术组大鼠中，PBF和IBF值均高于假手术组大鼠（两个值的p均小于0.001）。与注射生理盐水的动物相比，葡萄糖给药对假手术组或手术组大鼠的PBF均无影响。然而，与注射生理盐水的假手术组大鼠相比，葡萄糖使假手术组大鼠的IBF显著增加（p小于0.01），而注射葡萄糖和注射生理盐水的手术组大鼠之间未观察到IBF有差异。假手术组大鼠中通过胰岛分流的PBF比例（fIBF）约为10%，手术组大鼠中约为20%。葡萄糖增加了假手术组大鼠的fIBF，但对手术组大鼠无影响。总之，在假手术组大鼠中，葡萄糖刺激的胰岛素释放伴随着IBF增加，但在手术组大鼠中未观察到这种情况。