采用 S. mutans 生物膜模型来评估抗菌物质和 enamel demineralization。
S. mutans biofilm model to evaluate antimicrobial substances and enamel demineralization.
机构信息
Piracicaba Dental School, University of Campinas, Piracicaba, São Paulo, Brazil.
出版信息
Braz Oral Res. 2010 Apr-Jun;24(2):135-41. doi: 10.1590/s1806-83242010000200002.
The aim of this study was to validate a model of S. mutans biofilm formation, which simulated 'feast-famine' episodes of exposure to sucrose that occur in the oral cavity, showed dose-response susceptibility to antimicrobials and allowed the evaluation of substances with anticaries potential. S. mutans UA159 biofilms were grown for 5 days on bovine enamel slabs at 37 degrees C, 10% CO2. To validate the model, the biofilms were treated 2x/day with chlorhexidine digluconate (CHX) at 0.012, 0.024 and 0.12% (concentration with recognized anti-plaque effect) and 0.05% NaF (concentration with recognized anti-caries effect). CHX showed dose-response effect decreasing biomass, bacterial viability and enamel demineralization (p < 0.05). Whereas, 0.05% NaF did not show antimicrobial effect but had similar effect to that of 0.12% CHX decreasing enamel demineralization (p < 0.05). The model developed has potential to evaluate the effect of substances on biofilm growth and on enamel demineralization.
本研究旨在验证一种变异链球菌生物膜形成模型，该模型模拟了口腔中蔗糖暴露的“饱食-饥饿”循环，对抗菌药物表现出剂量反应敏感性，并可用于评估具有抗龋潜力的物质。将变异链球菌 UA159 生物膜在 37°C、10%CO2 下于牛牙釉质板上培养 5 天。为了验证该模型，生物膜每天用葡萄糖酸氯己定（CHX）处理 2 次，浓度分别为 0.012%、0.024%和 0.12%（具有公认的抗菌斑作用）和 0.05%氟化钠（具有公认的抗龋作用）。CHX 表现出剂量反应效应，降低生物量、细菌活力和牙釉质脱矿（p<0.05）。然而，0.05%氟化钠没有表现出抗菌作用，但与 0.12%CHX 相似，降低牙釉质脱矿（p<0.05）。所开发的模型具有评估物质对生物膜生长和牙釉质脱矿影响的潜力。
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