Department of Children's Clinic, Ribeirão Preto School of Dentistry, University of São Paulo, Café Avenue s/n, Ribeirão Preto, São Paulo, 14040-904, Brazil.
Department of BioMolecular Sciences, School of Pharmaceutical Sciences of Ribeirão Preto, University of São Paulo, Café Avenue s/n, Ribeirão Preto, São Paulo, 14040-903, Brazil.
Curr Microbiol. 2023 Aug 5;80(9):312. doi: 10.1007/s00284-023-03417-7.
This study aimed to evaluate the disruptive effect of fungal mutanase against cariogenic biofilm after short-term treatment. For that, mature Streptococcus mutans biofilms (n = 9) were exposed to active or inactivated enzymes produced by Trichoderma harzianum for 1 min, two times per day. Biofilms were analyzed by amount of matrix water-insoluble polysaccharides, bacterial viability, acidogenicity, and morphology by scanning electron microscopy (SEM). The group treated with active enzymes (AE) had a significantly lower amount of insoluble polysaccharides (893.30 ± 293.69) when compared to the negative control group (NaCl, 2192.59 ± 361.96), yet no significant difference was found when comparing to the positive control group (CHX, 436.82 ± 151.07). Also, there was no significant effect on bacteria metabolism and viability (P-value < 0.05). Data generated by the quantitative analysis were confirmed through scanning electron microscopy images. Thus, fungal mutanase degraded the biofilm after a short-term treatment without interfering with bacterial viability and metabolism. Such findings offer insight to the development of routine oral care products containing this input.
本研究旨在评估真菌突变酶对致龋生物膜的短期治疗后的破坏作用。为此，将成熟的变形链球菌生物膜（n=9）暴露于哈茨木霉产生的活性或失活酶中，每天两次，每次 1 分钟。通过扫描电子显微镜（SEM）分析基质水不溶性多糖的量、细菌活力、产酸和形态。与阴性对照组（NaCl，2192.59±361.96）相比，用活性酶（AE）处理的组的不溶性多糖量（893.30±293.69）显著降低，但与阳性对照组（CHX，436.82±151.07）相比无显著差异。此外，细菌代谢和活力也没有显著影响（P 值<0.05）。定量分析生成的数据通过扫描电子显微镜图像得到证实。因此，真菌突变酶在短期治疗后降解了生物膜，而不干扰细菌活力和代谢。这些发现为开发含有这种成分的常规口腔护理产品提供了思路。
