Institute of Applied Ecology, Chinese Academy of Sciences, No.72 Wenhua Road, Shenyang 110016, People's Republic of China.
Biotechnol Lett. 2010 Nov;32(11):1693-7. doi: 10.1007/s10529-010-0339-0. Epub 2010 Jul 25.
We have developed a convenient Escherichia coli transformation method, termed colony-based transformation, in which a single fresh colony of plasmid-containing donor strain is used instead of extracted plasmid to transform E. coli recipient cell. Thus the need for plasmid extraction and competent cell preparation is avoided. Additionally, a high-throughput transformation process based on this method was designed in which samples are prepared in a 96-well PCR plate and hundreds of transformations can be performed simultaneously in a thermocycler. We therefore suggest that this method may serve as a substitute of current transformation methods based on plasmid extraction.
我们开发了一种便捷的大肠杆菌转化方法，称为菌落转化法，该方法使用含有质粒的供体菌的单个新鲜菌落代替提取的质粒转化大肠杆菌受体细胞。因此，避免了质粒提取和感受态细胞制备的需要。此外，基于该方法设计了高通量转化过程，其中样品在 96 孔 PCR 板中制备，并且可以在热循环仪中同时进行数百次转化。因此，我们建议该方法可以替代目前基于质粒提取的转化方法。
