Zhou Yong-jian, Ye Qin, Lu Hui-shan, Yang Ying-hong, Guan Guo-xian, Huang Chang-ming, Wang Chuan, Zhang Jie
Department of Pathology, Union Hospital, Fujian Medical University, Fuzhou 350001, China.
Zhonghua Wei Chang Wai Ke Za Zhi. 2010 Jul;13(7):516-9.
To investigate the possibility of microvessel density (MVD) and blood vessel invade (BVI) as the indexes in predicting prognosis of rectal carcinoma at stages I to II.
Tumor tissues from 380 patients who underwent resection of stage I or II rectal cancer were analyzed for MVD and BVI by immunohistochemical S-P method with anti-CD105 and anti-CD 34 antibody. Binary and multivariable Cox regression was applied to indicate independent factors associated with overall survival.
CD105 was present in the neovascularity of the cancer tissue but not in the normal tissue, while CD34 was present in the tumor tissue and the normal tissue. BVI on CD34 staining was significantly higher than that on HE staining. Multivariable analysis revealed that TNM stage, CD34-BVI, histologic type, and CD105-MDV were independent risk factors to predict the possibility of poor prognosis of stage I or II rectal cancer. CD34-BVI or CD105-MVD positivity had a hazard ratio of 4.483 (95% confidence interval 2.861-7.026) for mortality.
The expressions of CD34-BVI and CD105-MVD are independent factors to predict the possibility of poor survival of stage I or II rectal carcinoma. Detection of CD105-MVD combined with CD34-BVI may help predict clinical outcome and design further individualized adjuvant treatment.
探讨微血管密度（MVD）和血管侵犯（BVI）作为预测Ⅰ至Ⅱ期直肠癌预后指标的可能性。
采用抗CD105和抗CD34抗体的免疫组织化学S-P法，对380例行Ⅰ期或Ⅱ期直肠癌切除术患者的肿瘤组织进行MVD和BVI分析。应用二元和多变量Cox回归分析以确定与总生存相关的独立因素。
CD105存在于癌组织的新生血管中，而在正常组织中不存在，而CD34在肿瘤组织和正常组织中均有表达。CD34染色的BVI显著高于苏木精-伊红（HE）染色。多变量分析显示，TNM分期、CD34-BVI、组织学类型和CD105-MDV是预测Ⅰ期或Ⅱ期直肠癌预后不良可能性的独立危险因素。CD34-BVI或CD105-MVD阳性患者的死亡风险比为4.483（95%置信区间2.861-7.026）。
CD34-BVI和CD105-MVD的表达是预测Ⅰ期或Ⅱ期直肠癌生存不良可能性的独立因素。检测CD105-MVD联合CD34-BVI可能有助于预测临床结局并设计进一步的个体化辅助治疗。
