Kiyama H, Wu J C, Smith M W, Lawson E D, Emson P C
MRC Groups, AFRC Institute of Animal Physiology and Genetics Research, Babraham, Cambridge, UK.
Differentiation. 1991 Mar;46(2):69-75. doi: 10.1111/j.1432-0436.1991.tb00867.x.
In situ hybridization and immunocytochemical techniques have been used to examine the distribution of vitamin-D-induced calbindin mRNA and calbindin protein in enterocytes lining the crypts and villi of chicken small intestine. Basal villus enterocytes contained approximately twice as much calbindin but over three times as much calbindin mRNA compared to values found in basal crypt and upper villus enterocytes, all values being measured 2 days after vitamin D injection into D-deficient chickens. Virtually no calbindin mRNA was detected in tissues taken from control D-deficient birds. Direct proportionality found between calbindin mRNA and calbindin content in enterocytes of basal crypt, mid and upper villus suggests pre-translational control over calbindin synthesis. The implications of possible inefficient translation of calbindin mRNA in basal villus enterocytes are discussed. Present methods of analysis provide a novel way to study mechanisms controlling gene expression throughout the whole process of enterocyte differentiation.
原位杂交和免疫细胞化学技术已被用于检测维生素D诱导的钙结合蛋白mRNA和钙结合蛋白在鸡小肠隐窝和绒毛的肠上皮细胞中的分布。与维生素D缺乏的鸡注射维生素D 2天后在隐窝基部和绒毛上部肠上皮细胞中所发现的值相比，绒毛基部肠上皮细胞中的钙结合蛋白含量约为其两倍，而钙结合蛋白mRNA含量则超过其三倍。实际上，在来自对照维生素D缺乏鸟类的组织中未检测到钙结合蛋白mRNA。在隐窝基部、绒毛中部和上部的肠上皮细胞中，钙结合蛋白mRNA与钙结合蛋白含量之间呈直接比例关系，这表明对钙结合蛋白合成存在转录前调控。讨论了绒毛基部肠上皮细胞中钙结合蛋白mRNA翻译可能效率低下的影响。目前的分析方法为研究在肠上皮细胞分化的整个过程中控制基因表达的机制提供了一种新途径。
