Department of Biomedical Science, CHA University, Seoul 135-081, Republic of Korea.
FEBS Lett. 2010 Aug 20;584(16):3629-34. doi: 10.1016/j.febslet.2010.07.037. Epub 2010 Jul 24.
Nobox is an oocyte-specific transcriptional regulator. Nobox deficiency disrupts early folliculogenesis and the expression of oocyte-specific genes in mice. In the present study, we found that peptidylarginine deiminase 6 (Pad6) was downregulated in Nobox-null ovaries. Pad6 is preferentially expressed in oocytes and its transcript is detectable at embryonic day 16.5. In addition, we identified one Nobox DNA-binding element (NBE) within the mouse Pad6 promoter. The NBE includes a core sequence TAATTA. Sequence-specific binding of Nobox to the TAATTA motif was confirmed. Nobox overexpression augmented transcriptional activity of a luciferase reporter driven by mouse Pad6. Our findings indicate that Nobox is a critical regulator that orchestrates oocyte-specific genes such as Pad6 during folliculogenesis.
Nobox 是一种卵母细胞特异性转录调节剂。Nobox 缺乏会破坏小鼠早期卵泡发生和卵母细胞特异性基因的表达。在本研究中，我们发现肽基精氨酸脱亚氨酶 6（Pad6）在 Nobox 缺失的卵巢中下调。Pad6 在卵母细胞中优先表达，其转录本在胚胎第 16.5 天即可检测到。此外，我们在小鼠 Pad6 启动子中鉴定出一个 Nobox DNA 结合元件（NBE）。该 NBE 包含一个核心序列 TAATTA。已证实 Nobox 与 TAATTA 基序的序列特异性结合。Nobox 的过表达增强了由小鼠 Pad6 驱动的荧光素酶报告基因的转录活性。我们的研究结果表明，Nobox 是一种关键的调节因子，它在卵泡发生过程中协调如 Pad6 等卵母细胞特异性基因的表达。
