Unité de Recherche 103, Centre IRD de Nouméa, Promenade Roger Laroque, BP A5 98848 Nouméa Cedex, Nouvelle Calédonie, France.
Mar Pollut Bull. 2010;61(7-12):530-41. doi: 10.1016/j.marpolbul.2010.06.038. Epub 2010 Jul 24.
The removal of nickel in marine tropical sediments (New Caledonia) was studied in microcosms. Removal of Ni(2+) was strongly enhanced by the presence of bacteria, with rates up to twofold higher than those observed under sterilized conditions. After 8 days of incubation, Ni(2+) concentration in the water column ranged from 30% to 50% of the initial concentration according to sediment origin. Addition of glucose stimulated bacterial processes and resulted in a complete disappearance of Ni(2+) in the water phase. Incubation under anoxic conditions slightly affects the microbial structure inferred from T-RFLP analysis irrespective of Ni(2+) spiking, whereas incubation under oxic conditions resulted to moderate modification of the microbial structure, changes that might be more marked in the presence of Ni(2+). Five different T-RFs were observed in almost all microcosms with relative abundance between 5% and 30%. Incubation with glucose resulted in the dominance of a common T-RF, with relative abundance up to 39%.
我们在微宇宙中研究了海洋热带沉积物（新喀里多尼亚）中镍的去除情况。在有细菌存在的情况下，Ni(2+)的去除率得到了极大的提高，比在灭菌条件下观察到的去除率高出两倍以上。在 8 天的孵育期内，根据沉积物的来源，水柱中 Ni(2+)的浓度范围为初始浓度的 30%至 50%。添加葡萄糖刺激了细菌的新陈代谢过程，导致水相中 Ni(2+)完全消失。在缺氧条件下孵育，无论是否添加 Ni(2+)，都对 T-RFLP 分析推断的微生物结构产生轻微影响，而在好氧条件下孵育则导致微生物结构的适度改变，在存在 Ni(2+)的情况下，这种改变可能更为明显。在几乎所有的微宇宙中都观察到了 5 种不同的 T-RF，相对丰度在 5%至 30%之间。用葡萄糖孵育导致一个常见的 T-RF 占主导地位，相对丰度高达 39%。
