丙酮酸对牛心线粒体介导的高铁肌红蛋白还原的影响。
Effects of pyruvate on bovine heart mitochondria-mediated metmyoglobin reduction.
机构信息
Department of Animal Science, University of Connecticut, Storrs, Connecticut 06269-4040, United States.
出版信息
Meat Sci. 2010 Nov;86(3):738-41. doi: 10.1016/j.meatsci.2010.06.014. Epub 2010 Jun 20.
Pyruvate can regenerate NADH through a variety of biochemical processes. However, no meat science research has determined if NADH produced via pyruvate can be used for mitochondria-mediated metmyoglobin reduction. Thus, our objectives were to assess the effects of pyruvate on mitochondria isolated from bovine cardiac muscle: oxygen consumption and metmyoglobin reduction at pH 5.6 and 7.4, 25°C in vitro. Both mitochondria and myoglobin were isolated from fresh bovine hearts (n=5). Mitochondria were reacted with pyruvate (50 mM), succinate (positive control; 50 mM), and antimycin A (mitochondrial inhibitor; 0.01 mM) and oxygen consumption was measured using a Clark oxygen electrode. Mitochondria (3 mg/mL) and metmyoglobin (0.15 mM) were reacted with either pyruvate, succinate, or antimycin A for 3h. Addition of succinate and pyruvate increased oxygen consumption and metmyoglobin reduction at pH 5.6 and 7.4 (succinate>pyruvate, P<0.05). Addition of a complex III inhibitor (antimycin A) decreased (P<0.05) oxygen consumption as well as metmyoglobin reduction associated with pyruvate and succinate. Results from the current study suggest that pyruvate can increase the ability of mitochondria to consume oxygen and reduce metmyoglobin.
丙酮酸可以通过多种生化过程再生 NADH。然而，肉类科学研究尚未确定通过丙酮酸产生的 NADH 是否可用于线粒体介导的高铁肌红蛋白还原。因此，我们的目的是评估丙酮酸对牛心肌分离的线粒体的影响：在 25°C、pH 值为 5.6 和 7.4 时的体外耗氧量和高铁肌红蛋白还原。线粒体和肌红蛋白均从新鲜牛心（n=5）中分离得到。用丙酮酸（50 mM）、琥珀酸（阳性对照；50 mM）和抗霉素 A（线粒体抑制剂；0.01 mM）处理线粒体，并使用克拉克氧电极测量耗氧量。将线粒体（3 mg/mL）和高铁肌红蛋白（0.15 mM）与丙酮酸、琥珀酸或抗霉素 A 反应 3 小时。在 pH 值为 5.6 和 7.4 时，琥珀酸和丙酮酸的添加均增加了耗氧量和高铁肌红蛋白还原（琥珀酸>丙酮酸，P<0.05）。添加复合物 III 抑制剂（抗霉素 A）会降低（P<0.05）与丙酮酸和琥珀酸相关的耗氧量和高铁肌红蛋白还原。本研究的结果表明，丙酮酸可以提高线粒体消耗氧气和还原高铁肌红蛋白的能力。
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