逆电子传递对 NADH 形成和高铁肌红蛋白还原的影响。
Reverse electron transport effects on NADH formation and metmyoglobin reduction.
机构信息
Department of Animal Science, University of Connecticut, Storrs, CT 06269, USA.
Department of Animal Science, Oklahoma State University, Stillwater, OK 74078, USA.
出版信息
Meat Sci. 2015 Jul;105:89-92. doi: 10.1016/j.meatsci.2015.02.012. Epub 2015 Mar 3.
The objective was to determine if NADH generated via reverse electron flow in beef mitochondria can be used for electron transport-mediated reduction and metmyoglobin reductase pathways. Beef mitochondria were isolated from bovine hearts (n=5) and reacted with combinations of succinate, NAD, and mitochondrial inhibitors to measure oxygen consumption and NADH formation. Mitochondria and metmyoglobin were reacted with succinate, NAD, and mitochondrial inhibitors to measure electron transport-mediated metmyoglobin reduction and metmyoglobin reductase activity. Addition of succinate and NAD increased oxygen consumption, NADH formation, electron transport-mediated metmyoglobin reduction, and reductase activity (p<0.05). Addition of antimycin A prevented electron flow beyond complex III, therefore, decreasing oxygen consumption and electron transport-mediated metmyoglobin reduction. Addition of rotenone prevented reverse electron flow, increased oxygen consumption, increased electron transport-mediated metmyoglobin reduction, and decreased NADH formation. Succinate and NAD can generate NADH in bovine tissue postmortem via reverse electron flow and this NADH can be used by both electron transport-mediated and metmyoglobin reductase pathways.
目的在于确定牛线粒体中逆向电子流产生的 NADH 是否可用于电子传递介导的还原和高铁肌红蛋白还原酶途径。从牛心（n=5）中分离牛线粒体，并与琥珀酸盐、NAD 和线粒体抑制剂组合反应，以测量耗氧量和 NADH 的生成。将线粒体和高铁肌红蛋白与琥珀酸盐、NAD 和线粒体抑制剂反应，以测量电子传递介导的高铁肌红蛋白还原和高铁肌红蛋白还原酶活性。添加琥珀酸盐和 NAD 可增加耗氧量、NADH 的生成、电子传递介导的高铁肌红蛋白还原和还原酶活性（p<0.05）。添加抗霉素 A 可阻止复合物 III 之后的电子流，从而减少耗氧量和电子传递介导的高铁肌红蛋白还原。添加鱼藤酮可阻止逆向电子流，增加耗氧量，增加电子传递介导的高铁肌红蛋白还原，并减少 NADH 的生成。琥珀酸盐和 NAD 可在牛组织死后通过逆向电子流产生 NADH，并且这种 NADH 可被电子传递介导和高铁肌红蛋白还原酶途径同时使用。
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