Mooney N, Van Alewyk D, Brick-Ghannan C, Charron D
Institut Biomédical des Cordeliers, Paris, France.
Int J Cancer Suppl. 1991;6:30-3. doi: 10.1002/ijc.2910470709.
Signal transduction via HLA class-II antigens has been studied using human resting B lymphocytes and monoclonal anti-HLA class-II antibodies. An increased intracellular calcium flux, phosphatidylinositol biphosphate hydrolysis and activation of protein kinase C have all been observed following signal transduction via HLA-class-II molecules. The interaction of HLA-class-II-mediated signalling with sIg-mediated signalling has been studied using a non-mitogenic anti-sIg. This combination provides a model for T-cell-dependent antigenic stimulation. The results demonstrate that stimulation via HLA class-II antigens permits a proliferative response to an otherwise non-mitogenic anti-sIg and that this effect is not restricted to one HLA class-II isotype. These data suggest that HLA-class-II-mediated signalling is important in responses to T-cell-dependent antigens.
利用人静止B淋巴细胞和抗HLA - II类单克隆抗体，对经由HLA - II类抗原的信号转导进行了研究。经由HLA - II类分子进行信号转导后，均观察到细胞内钙通量增加、磷脂酰肌醇二磷酸水解以及蛋白激酶C激活。使用非促有丝分裂抗sIg研究了HLA - II类介导的信号传导与sIg介导的信号传导之间的相互作用。这种组合为T细胞依赖性抗原刺激提供了一个模型。结果表明，经由HLA - II类抗原的刺激允许对原本无促有丝分裂作用的抗sIg产生增殖反应，并且这种效应不限于一种HLA - II类同种型。这些数据表明，HLA - II类介导的信号传导在对T细胞依赖性抗原的反应中很重要。
