hnRNP A1 与辛德毕斯病毒的基因组和亚基因组 RNA 启动子相互作用，是 G 和 SG RNA 合成所必需的。
hnRNP A1 interacts with the genomic and subgenomic RNA promoters of Sindbis virus and is required for the synthesis of G and SG RNA.
机构信息
Department of Molecular Genetics, Microbiology & Immunology, UMDNJ-Robert Wood Johnson Medical School, Piscataway, NJ 08854, USA.
出版信息
J Biomed Sci. 2010 Jul 21;17(1):59. doi: 10.1186/1423-0127-17-59.
BACKGROUND
Sindbis virus (SV) is the prototype of alphaviruses which are a group of widely distributed human and animal pathogens. Heterogeneous nuclear ribonucleoprotein (hnRNP) A1 is an RNA-binding protein that shuttles between the nucleus and the cytoplasm. Our recent studies found that hnRNP A1 relocates from nucleus to cytoplasm in Sindbis virus (SV)-infected cells. hnRNP A1 binds to the 5' UTR of SV RNA and facilitates the viral RNA replication and translation.
METHODS
Making use of standard molecular techniques, virology methods and an in vitro system developed by our lab to assess the role of hnRNP A1 in SV positive strand RNA synthesis.
RESULTS
hnRNP A1 interacted with the genomic (G) and subgenomic (SG) RNA promoters. Knockdown of hnRNP A1 resulted in markedly decrease in the synthesis of G and SG RNA both in infected cells and in vitro.
CONCLUSIONS
Our study provides the first direct evidence that hnRNP A1 actively participates in viral RNA replication and is required for the synthesis of G and SG RNA.
背景
辛德毕斯病毒（SV）是甲病毒属的原型病毒，该属病毒是一组广泛分布于人类和动物中的病原体。异质核核糖核蛋白（hnRNP）A1 是一种 RNA 结合蛋白，可在核和细胞质之间穿梭。我们最近的研究发现，在辛德毕斯病毒（SV）感染的细胞中，hnRNP A1 从核内重新分布到细胞质中。hnRNP A1 与 SV RNA 的 5'UTR 结合，促进病毒 RNA 的复制和翻译。
方法
利用标准分子技术、病毒学方法和我们实验室开发的体外系统，评估 hnRNP A1 在 SV 正链 RNA 合成中的作用。
结果
hnRNP A1 与基因组（G）和亚基因组（SG）RNA 启动子相互作用。hnRNP A1 的敲低导致感染细胞和体外 G 和 SG RNA 的合成明显减少。
结论
我们的研究首次直接证明 hnRNP A1 积极参与病毒 RNA 复制，是 G 和 SG RNA 合成所必需的。
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