Ye J, Nie W, Wang J, Su W, Jing M, Graphodatsky A S, Yang F
State Key Laboratory of Genetic Resources and Evolution, Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming, PR China.
Cytogenet Genome Res. 2011;132(1-2):41-6. doi: 10.1159/000317082. Epub 2010 Jul 24.
Comparative genomic data for ochotonids (pikas) are important for understanding the karyotype evolution of lagomorphs. Here, we have established the first genome-wide chromosomal homologies between human and the Forrest's pika (Ochotona forresti, 2n = 54) by cross-species chromosome painting. Integration of our map with the published comparative chromosome map between human and rabbit has enabled the establishment of an indirect homology map between Forrest's pika and domestic rabbit (Oryctolagus cuniculus, 2n = 44), representing the 2 Lagomorpha families: Leporidae and Ochotonidae. Our results demonstrate that (1) most of the proposed ancestral eutherian syntenies or syntenic associations have been retained in the O. forresti genome; (2) the HSA1/10p association, one of the 2 signature rearrangements that were proposed to support the grouping of the orders Lagomorpha and Rodentia into a monophyletic clade called Glires, is also present in the O. forresti genome; and (3) Robertsonian translocations have contributed to the karyotype differences between O. forresti and O. cuniculus.
鼠兔类（鼠兔）的比较基因组数据对于理解兔形目动物的核型进化很重要。在此，我们通过跨物种染色体涂染建立了人类与高原鼠兔（Ochotona forresti，2n = 54）之间首个全基因组染色体同源性图谱。将我们的图谱与已发表的人类和家兔之间的比较染色体图谱整合，得以建立高原鼠兔与家兔（Oryctolagus cuniculus，2n = 44）之间的间接同源性图谱，代表了兔形目两个科：兔科和鼠兔科。我们的结果表明：（1）大多数推测的原始真兽类同线性或同线性关联在高原鼠兔基因组中得以保留；（2）人类1号染色体与10号染色体短臂的关联，这是为支持将兔形目和啮齿目归为一个单系类群即啮齿动物而提出的两个标志性重排之一，在高原鼠兔基因组中也存在；（3）罗伯逊易位导致了高原鼠兔和家兔之间的核型差异。
