Colbert D L, Eremin S A, Landon J
Department of Chemical Pathology, St. Bartholomew's Hospital, London, U.K.
J Immunol Methods. 1991 Jul 5;140(2):227-33. doi: 10.1016/0022-1759(91)90375-p.
Small haptens such as methylamphetamine (MW 149) cannot, on their own, induce an immune response. It is also unlikely that they fill the binding site of any antibody that recognises them. Under such circumstances any attached label might be expected to enter the area of the binding site and exert an influence on overall binding. To investigate the possible influence of the label on binding, a range of fluorescein-labelled derivatives, differing in bridge length, were prepared. Antiserum binding of these labelled derivatives was then compared to that of the unlabelled drug. Evidence is presented which suggests that, with small haptens, the closeness of the fluorescein molecule can markedly influence antibody binding. Significant differences were found in titre, sensitivity, and assay kinetics. These overall effects appear to be brought about by the change in affinity of the antibody for the labelled hapten.
诸如甲基苯丙胺（分子量149）之类的小分子半抗原自身无法诱导免疫反应。它们也不太可能占据识别它们的任何抗体的结合位点。在这种情况下，任何附着的标记物可能会进入结合位点区域并对整体结合产生影响。为了研究标记物对结合的可能影响，制备了一系列桥长不同的荧光素标记衍生物。然后将这些标记衍生物的抗血清结合情况与未标记药物的抗血清结合情况进行比较。所提供的证据表明，对于小分子半抗原，荧光素分子的接近程度会显著影响抗体结合。在滴度、灵敏度和测定动力学方面发现了显著差异。这些总体效应似乎是由抗体对标记半抗原的亲和力变化引起的。
