Division of Viral Diseases, Centers for Disease Control and Prevention, Atlanta, GA, USA.
J Clin Virol. 2010 Sep;49(1):73-4. doi: 10.1016/j.jcv.2010.06.022. Epub 2010 Jul 27.
Molecular diagnostic tests to detect enterovirus in clinical specimens usually target highly conserved sites in the 5'-non-translated region, allowing detection of all members of the genus. The sequences in the 5'-NTR do not correlate with serotype, but PCR and sequencing of the VP1 region can be used for typing; this system has largely replaced traditional antigenic typing.
To investigate the relative performance of two common enterovirus assays.
We compared the relative sensitivities of Taqman real-time RT-PCR (rRT-PCR) and a VP1 semi-nested PCR (RT-snPCR) assay in which sequencing the VP1 amplicon also provides typing information.
There was 89% concordance between the two methods among the 371 clinical specimens tested (74 positive in both assays and 257 negative in both assays). Twenty-seven rRT-PCR-negative/VP1-positive specimens were confirmed positive by sequencing; 13 specimens were rRT-PCR-positive/VP1-negative.
The results suggest that either assay can produce satisfactory results, but that using both assays in parallel should provide the highest sensitivity for clinical diagnostic testing.
用于检测临床标本中肠道病毒的分子诊断测试通常针对 5'非翻译区中高度保守的位点，从而能够检测属内的所有成员。5'-NTR 中的序列与血清型无关，但可以使用 VP1 区的 PCR 和测序进行分型；该系统已在很大程度上取代了传统的抗原分型。
研究两种常用肠道病毒检测方法的相对性能。
我们比较了 Taqman 实时 RT-PCR（rRT-PCR）和 VP1 半巢式 PCR（RT-snPCR）检测方法的相对敏感性，其中对 VP1 扩增子进行测序也提供了分型信息。
在 371 份临床标本中，两种方法之间的一致性为 89%（两种检测方法均为阳性的有 74 份，均为阴性的有 257 份）。27 份 rRT-PCR 阴性/VP1 阳性的标本经测序后确认为阳性；13 份标本 rRT-PCR 阳性/VP1 阴性。
结果表明，两种检测方法均可获得满意的结果，但平行使用两种检测方法可提高临床诊断检测的灵敏度。
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