Poh Chit Laa, Tan Eng Lee
Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia.
Methods Mol Biol. 2011;665:65-77. doi: 10.1007/978-1-60761-817-1_5.
Enteroviruses are positive stranded RNA viruses belonging to the genus Enterovirus of the Picornaviridae family. Human enteroviruses are transmitted through the fecal-oral route and have been shown to cause mild to life-threatening diseases. Various diagnostic methods have been developed to detect enteroviruses from clinical specimens but many were impeded by requirements for special reagents, lengthy procedures, low sensitivity or cross-reactivity. This chapter describes rapid and highly sensitive methods of enteroviral detection directly from clinical specimens based on a conventional one-step Reverse Transcription polymerase chain reaction (RT-PCR) and a one-step real-time RT-PCR.
肠道病毒是正链RNA病毒，属于小RNA病毒科肠道病毒属。人肠道病毒通过粪口途径传播，已被证明可引起从轻度到危及生命的疾病。已开发出多种诊断方法来从临床标本中检测肠道病毒，但许多方法受到特殊试剂要求、程序冗长、灵敏度低或交叉反应性的阻碍。本章介绍了基于传统一步法逆转录聚合酶链反应（RT-PCR）和一步法实时RT-PCR直接从临床标本中快速、高灵敏度检测肠道病毒的方法。
