Liu Jiangtao, Tan Lijun, Li Huijun, Wang Qiwei, Ji Wenyue
Department of Otorhinolaryngology, China Medical University Affiliated Shengjing Hospital, Shenyang, China.
ORL J Otorhinolaryngol Relat Spec. 2010;72(4):205-14. doi: 10.1159/000314689. Epub 2010 Jul 29.
S-phase kinase-associated protein 2 (Skp2) is related to cellular proliferation and differentiation in laryngeal carcinoma. This study aimed to investigate the effect of Skp-2 suppression on p27 expression, cellular proliferation, apoptosis, and tumor growth in laryngeal squamous cell carcinoma. Skp2 was stably suppressed in Hep2 laryngeal carcinoma cells by lentivirus siRNA technology and Hep2-siRNA was then inoculated into nude mice. The siRNA-induced suppression of Skp2 increased p27 expression, decreased cellular proliferation, and increased apoptosis in human laryngeal carcinoma cells and tumors, indicating that Skp2 is a viable target in the gene therapy treatment of human laryngeal carcinoma.
S期激酶相关蛋白2（Skp2）与喉癌的细胞增殖和分化相关。本研究旨在探讨抑制Skp-2对喉鳞状细胞癌中p27表达、细胞增殖、凋亡及肿瘤生长的影响。通过慢病毒小干扰RNA（siRNA）技术在Hep2喉癌细胞中稳定抑制Skp2，然后将Hep2-siRNA接种到裸鼠体内。siRNA诱导的Skp2抑制增加了人喉癌细胞和肿瘤中p27的表达，降低了细胞增殖，并增加了细胞凋亡，表明Skp2是人类喉癌基因治疗的一个可行靶点。
