肿瘤组织中 CSNK1A1 和 AAC-11 的基因表达水平而非 NME1 可作为 NSCLC 患者的预后因素。

Gene expression levels of CSNK1A1 and AAC-11, but not NME1, in tumor tissues as prognostic factors in NSCLC patients.

机构信息

Tianjin Key Laboratory of Lung Cancer Metastasis and Tumor Microenvironment, Tianjin Lung Cancer Institute, Tianjin Medical University General Hospital, Tianjin, China.

出版信息

Med Sci Monit. 2010 Aug;16(8):CR357-64.

PMID:20671611

Abstract

BACKGROUND

To analyze the prognostic significance of the genes casein kinase 2 alpha subunit (CSNK2A1), anti-apoptosis clone-11 (AAC-11), and tumor metastasis suppressor NME1 in completely resected non-small cell lung cancer (NSCLC) patients.

MATERIAL/METHODS: Total RNA was extracted from 145 cases of completely resected, formalin-fixed, paraffin-embedded NSCLC tissues. mRNA expression levels of CSNK2A1, AAC-11, and NME1 were detected by real-time reverse-transcriptase polymerase chain reaction. Univariate and multivariate survival analyses were used to identify factors related to prognosis.

RESULTS

A correlation between CSNK2A1 and AAC-11 mRNA expression levels (rs=0.366, p=0.000) was found. Univariate analysis showed that high expression of CSNK2A1 and AAC-11 was predictive of poor prognosis in NSCLC patients (p=0.029 and 0.044, respectively), especially when expression levels of both genes were concomitantly high (p=0.007). Multivariate Cox regression analysis showed that high expression of CSNK2A1, or concomitantly high expression of CSNK2A1 and AAC-11, are independent prognostic factors of poor survival in NSCLC patients. However, NME1 mRNA expression level did not significantly influence survival in NSCLC patients.

CONCLUSIONS

This retrospective study indicates that CSNK2A1 and AAC-11, especially in combination, are useful prognosis markers in NSCLC patients after complete resection, independent of lymph node metastasis status.

摘要

背景

分析细胞周期蛋白依赖性激酶 2 样激酶 1（CSNK2A1）、凋亡抑制因子克隆-11（AAC-11）和肿瘤转移抑制因子 1（NME1）在完全切除的非小细胞肺癌（NSCLC）患者中的预后意义。

材料/方法：从 145 例完全切除、福尔马林固定、石蜡包埋的 NSCLC 组织中提取总 RNA。采用实时逆转录聚合酶链反应检测 CSNK2A1、AAC-11 和 NME1 的 mRNA 表达水平。采用单因素和多因素生存分析确定与预后相关的因素。

结果

发现 CSNK2A1 和 AAC-11 mRNA 表达水平之间存在相关性（rs=0.366，p=0.000）。单因素分析显示，CSNK2A1 和 AAC-11 高表达与 NSCLC 患者预后不良相关（p=0.029 和 0.044），尤其是当两种基因的表达水平同时升高时（p=0.007）。多因素 Cox 回归分析表明，CSNK2A1 高表达或 CSNK2A1 和 AAC-11 同时高表达是 NSCLC 患者不良生存的独立预后因素。然而，NME1 mRNA 表达水平对 NSCLC 患者的生存无显著影响。