Hennig Geoffrey S, Hosgood Giselle, Bubenik-Angapen Loretta J, Lauer Susanne K, Morgan Timothy W
Department of Veterinary Clinical Sciences, School of Veterinary Medicine, Louisiana State University, Baton Rouge, LA 70803, USA.
Am J Vet Res. 2010 Aug;71(8):875-83. doi: 10.2460/ajvr.71.8.875.
To evaluate chondrocyte death in canine articular cartilage exposed in vitro to bupivacaine with and without methylparaben and to compare viability for cartilage with intact or mechanically debrided surfaces.
Both glenohumeral joints from 10 adult canine cadavers.
10 osteochondral cores were harvested from each of the 20 humeral heads; synovium and 1 core from each joint were examined to verify joint health, and the other 9 cores were exposed to canine chondrocyte culture medium (CCCM), a 0.5% solution of bupivacaine, or 0.5% solution of bupivacaine with methylparaben for 5, 15, or 30 minutes.
For the superficial zone of surface-intact chondrocytes, bupivacaine with methylparaben caused a significantly higher percentage of chondrocyte death at 5 minutes (47.7%) than did bupivacaine (23.6%) or CCCM (25.4%). Bupivacaine (53.8%) and bupivacaine with methylparaben (62.5%) caused a significantly higher percentage of chondrocyte death at 30 minutes than did CCCM (20.0%). For the superficial zone of chondrocytes with debrided surfaces, bupivacaine with methylparaben caused a significantly higher percentage of chondrocyte death at 30 minutes (59%) than it did at 5 minutes (37.7%). Bupivacaine with methylparaben caused a significantly higher percentage of chondrocyte death at 30 minutes (59.0%) than did CCCM (28.9%). For middle and deep zones of chondrocytes, treatment solution and surface debridement had minimal effects on percentage of chondrocyte death.
Bupivacaine and bupivacaine with methylparaben were cytotoxic to canine articular chondrocytes in vitro. Intra-articular administration of bupivacaine is not recommended for clinical use until additional studies are conducted.
评估在体外暴露于布比卡因（含或不含对羟基苯甲酸甲酯）的犬关节软骨中的软骨细胞死亡情况，并比较完整或机械清创表面的软骨的活力。
10只成年犬尸体的双侧肩关节。
从20个肱骨头中各采集10个骨软骨芯；检查每个关节的滑膜和1个芯以确认关节健康状况，将其他9个芯暴露于犬软骨细胞培养基（CCCM）、0.5%布比卡因溶液或含对羟基苯甲酸甲酯的0.5%布比卡因溶液中5、15或30分钟。
对于表面完整的软骨细胞浅层区域，含对羟基苯甲酸甲酯的布比卡因在5分钟时导致的软骨细胞死亡百分比（47.7%）显著高于布比卡因（23.6%）或CCCM（25.4%）。布比卡因（53.8%）和含对羟基苯甲酸甲酯的布比卡因（62.5%）在30分钟时导致的软骨细胞死亡百分比显著高于CCCM（20.0%）。对于表面清创的软骨细胞浅层区域，含对羟基苯甲酸甲酯的布比卡因在30分钟时导致的软骨细胞死亡百分比（59%）显著高于5分钟时（37.7%）。含对羟基苯甲酸甲酯的布比卡因在30分钟时导致的软骨细胞死亡百分比（59.0%）显著高于CCCM（28.9%）。对于软骨细胞的中层和深层区域，处理溶液和表面清创对软骨细胞死亡百分比的影响极小。
布比卡因和含对羟基苯甲酸甲酯的布比卡因在体外对犬关节软骨细胞具有细胞毒性。在进行更多研究之前，不建议临床关节内注射布比卡因。
