University of California, Irvine School of Medicine, Irvine, CA, USA.
Department of Biomedical Engineering, University of California, Irvine, Irvine, CA, USA.
Cartilage. 2024 Jun;15(2):156-163. doi: 10.1177/19476035231164751. Epub 2023 Mar 29.
The purpose of this study was to determine the effects of a single exposure of bupivacaine on the mechanical properties of bovine cartilage explants at 3 weeks.
Femoral condyle articular cartilage explants were aseptically harvested from juvenile bovine stifle joints before being exposed to chondrogenic medium containing 0.50% (wt/vol) bupivacaine, 0.25% (wt/vol) bupivacaine, or no medication (control) for 1 hour. Explants were then washed and maintained in culture for 3 weeks before testing. Cell viability, tensile and compressive mechanical properties, histological properties, and biochemical properties were then assessed.
Explants exhibited a dose-dependent decrease in mean tensile Young's modulus with increasing bupivacaine concentration (9.86 MPa in the controls, 6.48 MPa in the 0.25% bupivacaine group [ = 0.048], and 4.72 MPa in the 0.50% bupivacaine group [ = 0.005]). Consistent with these results, collagen content and collagen crosslinking decreased with bupivacaine exposure as measured by mass spectrometry. Compressive properties of the explants were unaffected by bupivacaine exposure. Explants also exhibited a trend toward dose-dependent decreases in viability (51.2% for the controls, 47.3% for the 0.25% bupivacaine-exposed group, and 37.0% for the 0.50% bupivacaine-exposed group [ = 0.072]).
Three weeks after 1-hour bupivacaine exposure, the tensile properties of bovine cartilage explants were significantly decreased, while the compressive properties remained unaffected. These decreases in tensile properties corresponded with reductions in collagen content and crosslinking of collagen fibers. Physicians should be judicious regarding the intra-articular administration of bupivacaine in native joints.
本研究旨在确定单次布比卡因暴露对 3 周时牛软骨外植体机械性能的影响。
从小牛膝关节无菌采集股骨髁关节软骨外植体，然后将其暴露于含有 0.50%（重量/体积）布比卡因、0.25%（重量/体积）布比卡因或无药物（对照）的软骨形成培养基中 1 小时。然后将外植体洗涤并在培养中维持 3 周，然后进行测试。随后评估细胞活力、拉伸和压缩机械性能、组织学特性和生化特性。
外植体表现出与布比卡因浓度呈剂量依赖性的平均拉伸杨氏模量降低（对照组为 9.86 MPa，0.25%布比卡因组为 6.48 MPa[=0.048]，0.50%布比卡因组为 4.72 MPa[=0.005]）。与这些结果一致，通过质谱法测量，胶原蛋白含量和胶原蛋白交联随布比卡因暴露而降低。外植体的压缩性能不受布比卡因暴露的影响。外植体的活力也呈剂量依赖性降低趋势（对照组为 51.2%，0.25%布比卡因暴露组为 47.3%，0.50%布比卡因暴露组为 37.0%[=0.072]）。
在 1 小时布比卡因暴露 3 周后，牛软骨外植体的拉伸性能显著降低，而压缩性能保持不变。这些拉伸性能的降低与胶原蛋白含量的减少和胶原蛋白纤维的交联减少相对应。医生在关节内注射布比卡因治疗原生关节时应慎重。
