Jepson Rosanne E, Syme Harriet M, Markwell Peter, Miyazaki Masao, Yamashita Tetsuro, Elliott Jonathan
Department of Veterinary Clinical Science, Royal Veterinary College, North Mymms, Hatfield, Hertfordshire AL9 7TA, England.
Am J Vet Res. 2010 Aug;71(8):982-7. doi: 10.2460/ajvr.71.8.982.
To evaluate urine cauxin immunoreactivity in geriatric cats with variable plasma creatinine concentrations and proteinuria and to assess urinary cauxin-to-creatinine concentration ratio (UC/C) as a predictor of developing azotemia.
188 client-owned geriatric (>or= 9 years of age) cats.
A direct immunoassay was developed and validated for the quantification of urinary cauxin relative to a standard curve generated from a urine sample with high cauxin immunoreactivity. Relationships among UC/C, plasma creatinine concentration, and proteinuria were assessed. Nonazotemic cats were recruited and followed for 12 months. Urinary cauxin-to-creatinine concentration ratio was evaluated as a predictor of development of azotemia in these cats.
No relationship was evident between UC/C and plasma creatinine concentration. A weak positive correlation was identified between UC/C and urine protein-to-creatinine concentration ratio (r = 0.212). At entry to the longitudinal study, those cats that later developed azotemia had a UC/C that was significantly higher than in those remaining nonazotemic after 12 months.
The UC/C did not vary with severity of azotemia but appeared contributory to the feline urinary proteome. High UC/C values were predictive of the geriatric cats in our study developing azotemia. However, it seems unlikely that UC/C will provide additional information about the measurement of urine protein-to-creatinine concentration ratio as a biomarker for the development of azotemia in cats.
评估血浆肌酐浓度和蛋白尿情况各异的老年猫尿液中考辛的免疫反应性，并评估尿考辛与肌酐浓度比值（UC/C）作为氮质血症发生预测指标的价值。
188只客户拥有的老年猫（年龄≥9岁）。
开发并验证了一种直接免疫测定法，用于相对于由具有高考辛免疫反应性的尿液样本生成的标准曲线定量测定尿考辛。评估UC/C、血浆肌酐浓度和蛋白尿之间的关系。招募非氮质血症猫并随访12个月。评估尿考辛与肌酐浓度比值作为这些猫氮质血症发生预测指标的价值。
UC/C与血浆肌酐浓度之间无明显关系。UC/C与尿蛋白与肌酐浓度比值之间存在弱正相关（r = 0.212）。在纵向研究开始时，那些后来发生氮质血症的猫的UC/C显著高于12个月后仍保持非氮质血症的猫。
UC/C并不随氮质血症严重程度而变化，但似乎对猫的尿液蛋白质组有影响。在我们的研究中高UC/C值可预测老年猫发生氮质血症。然而，UC/C似乎不太可能为作为猫氮质血症发生生物标志物的尿蛋白与肌酐浓度比值测量提供额外信息。
