Department of Pathology, University of Pittsburgh Medical Center, Pittsburgh, PA, USA.
Vox Sang. 2010 Nov;99(4):325-31. doi: 10.1111/j.1423-0410.2010.01365.x.
The mechanical fragility index (MFI) is an in vitro measurement of the extent of RBC sublethal injury. Sublethal injury might constitute a component of the RBC storage lesion, thus the MFI was determined serially during routine RBC storage.
Leucoreduced AS-5- and SAGM-preserved RBCs were stored under routine blood bank conditions. The mechanical fragility (MF) of each unit was serially measured during storage.
For both AS-5 and SAGM units, male and female RBCs demonstrated statistically significant increases in the MFI during storage. The MFI was significantly lower in AS-5 units compared to SAGM units throughout storage. Female RBCs had significantly lower MFI vs. male RBCs in both AS-5 and SAGM units at all times. No significant differences in MFI were observed between ABO groups for both genders for AS-5 RBCs.
The MF of RBCs increases during storage. Both gender and preservation solution influenced the MFI; however, the male:female MFI ratios were similar at all time-points and remained stable, suggesting that gender-based biological differences exist independent of storage solution. The MF could be a useful test for evaluating the effect of novel interventions intended to mitigate the susceptibility of RBCs to sublethal injury during storage.
机械脆弱指数（MFI）是体外测量 RBC 亚致死损伤程度的指标。亚致死损伤可能构成 RBC 储存损伤的一个组成部分，因此在常规 RBC 储存过程中连续测定 MFI。
使用白细胞减少的 AS-5 和 SAGM 保存的 RBC 在常规血库条件下储存。在储存过程中连续测量每个单位的机械脆性（MF）。
对于 AS-5 和 SAGM 单位，男性和女性 RBC 的 MFI 在储存过程中均呈统计学显著增加。与 SAGM 单位相比，AS-5 单位的 MFI 在整个储存过程中显著降低。在 AS-5 和 SAGM 单位中，女性 RBC 的 MFI 始终低于男性 RBC。对于 AS-5 RBC，ABO 组之间的 MFI 无性别差异。
RBC 的 MF 在储存过程中增加。性别和保存液都影响 MFI；然而，在所有时间点，男性和女性的 MFI 比值相似且保持稳定，这表明基于性别的生物学差异独立于保存液。MF 可能是一种有用的测试方法，用于评估旨在减轻 RBC 在储存过程中对亚致死损伤易感性的新型干预措施的效果。
