Institute of Biochemistry, Mokslininku 12, LT-08662 Vilnius, Lithuania.
Biosens Bioelectron. 2010 Oct 15;26(2):766-71. doi: 10.1016/j.bios.2010.06.038. Epub 2010 Jul 1.
The pyrroloquinoline quinone (PQQ)-dependent soluble glucose dehydrogenase based carbon paste electrodes were investigated and applied for glucose monitoring in the oxygen deficient media. Reagentless biosensors possessing a wide linear range (up to 5 mM glucose with a detection limit of 0.12 mM) were designed. The oxygen-insensitive response of the biosensor creates the opportunity to use it as a flow-through device for continuous monitoring of glucose in media during the wine yeast fermentation process. The analysis of glucose assimilation rate by yeast strains using the developed biosensor correlated well (R2=0.9938) with convenient yeast testing methods.
研究了依赖吡咯并喹啉醌（PQQ）的可溶性葡萄糖脱氢酶碳糊电极，并将其应用于缺氧介质中的葡萄糖监测。设计了无试剂生物传感器，具有较宽的线性范围（高达 5 mM 葡萄糖，检测限为 0.12 mM）。生物传感器对氧气不敏感的响应为其提供了机会，使其可作为一种流通式装置，用于在葡萄酒酵母发酵过程中连续监测介质中的葡萄糖。使用开发的生物传感器分析酵母菌株的葡萄糖同化率与方便的酵母测试方法相关性良好（R2=0.9938）。
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