College of Materials Science and Engineering, Shenzhen University, and Shenzhen Key Laboratory of Special Functional Materials, Shenzhen 518060, PR China.
Biosens Bioelectron. 2010 Sep 15;26(1):295-8. doi: 10.1016/j.bios.2010.06.036. Epub 2010 Jun 30.
Carbon nanotubes (CNTs) were "dissolved" in mixed solution of cyclodextrin (CD) and cyclodextrin prepolymer (pre-CDP) and were used as modifier to fabricate chemical modified electrode. The dispersions of CNTs in different solutions were characterized using UV-vis spectrophotometer. The insoluble conducting composite film of poly-cyclodextrin (CDP) and carbon nanotube was synthesized, and glucose oxidase (GOx) was immobilized on the film to fabricate amperometric biosensor. The CNT-CDP electrode was stable. It can keep the exceptional chemical and physical properties of CNTs and the host-guest chemical reaction ability of cyclodextrins. Cyclic voltammetry measurements of potassium ferricyanide solution (50 mM, and scan rate 100 mVs(-1)) shows that the CDP film was compact and the CNT-CDP film maintains the electrocatalytic activity of CNT. Glucose oxidase was used as a model enzyme to prepare a glucose biosensor. The bioactivity of immobilized glucose oxidase was maintained due to the biocompatibility of cyclodextrin. Amperometric measurements were done with different concentrations of glucose. The CNT-CDP/GCE-GOx biosensor has wide concentration ranges and good sensitivity to glucose. It showed a detection limit of 3.5 μM with a linear range from 0.004 to 3.23 mM and from 4.26 to 10.00 mM. In addition, the biosensor can be operated under wide pH range (pH 5.6-7.8) without great changes in its sensitivity.
碳纳米管（CNTs）“溶解”在环糊精（CD）和环糊精预聚物（pre-CDP）的混合溶液中，并用作修饰剂来制备化学修饰电极。使用紫外-可见分光光度计对 CNTs 在不同溶液中的分散情况进行了表征。合成了聚环糊精（CDP）和碳纳米管的不溶性导电复合膜，并将葡萄糖氧化酶（GOx）固定在膜上制备电流型生物传感器。 CNT-CDP 电极稳定，既能保持 CNTs 的特殊化学和物理性质，又能保持环糊精的主客体化学反应能力。对 50mM 的铁氰化钾溶液（扫描速率为 100mVs(-1)）进行循环伏安法测量表明，CDP 膜致密，且 CNT-CDP 膜保持 CNT 的电催化活性。使用葡萄糖氧化酶作为模型酶制备葡萄糖生物传感器。由于环糊精的生物相容性，固定化葡萄糖氧化酶的生物活性得以保持。对不同浓度的葡萄糖进行了电流测量。CNT-CDP/GCE-GOx 生物传感器对葡萄糖具有较宽的浓度范围和良好的灵敏度，检测限为 3.5μM，线性范围为 0.004-3.23mM，4.26-10.00mM。此外，该生物传感器可在较宽的 pH 范围（5.6-7.8）下工作，其灵敏度无明显变化。
