正畸手术后咀嚼肌中发育型肌球蛋白重链 mRNA 的表达：一项初步研究。
Developmental myosin heavy chain mRNA in masseter after orthognathic surgery: a preliminary study.
机构信息
Department of Orthodontics, Medical Faculty Carl Gustav Carus, Technical University of Dresden, Germany.
出版信息
J Craniomaxillofac Surg. 2011 Sep;39(6):401-6. doi: 10.1016/j.jcms.2010.06.001. Epub 2010 Jul 29.
INTRODUCTION
A microarray study showed an increase of developmental myosin heavy chain (MyHC) mRNA in the masseter muscle after surgery. The aim of the study was to determine the expression of the embryonic MYH3 and perinatal MYH8 for use as potential marker for muscle adaptation after orthognathic surgery.
MATERIAL AND METHODS
24 adult patients with a prognathic (11) or retrognathic (13) mandible were involved in the study. 192 biopsies were taken from two parts of the muscles presurgically and 6 months following. The expression of MYH3 and MYH8 were quantified using real-time polymerase chain reaction (RT-PCR). Absolute quantification was done by CT-values.
RESULTS
MYH3 was up-regulated in prognathia (8.5, P<0.001) and in retrognathia (2.8, P<0.043). MYH8 was up-regulated in retrognathia only (4.0, P<0.063) and down-regulated in prognathia (-1.6). MYH3 values correlated in retrognathic patients (P<0.005) before and after surgery.
CONCLUSION
MYH3 and MYH8 could play a role in functional adaptation after orthognathic surgery and orofacial orthopaedics.
简介
一项微阵列研究显示，手术后咀嚼肌中的发育性肌球蛋白重链（MyHC）mRNA 增加。本研究的目的是确定胚胎 MYH3 和围产期 MYH8 的表达，作为正颌手术后肌肉适应的潜在标志物。
材料和方法
24 名下颌前突（11 名）或后缩（13 名）的成年患者参与了本研究。在术前和术后 6 个月，从肌肉的两个部位采集了 192 个活检样本。使用实时聚合酶链反应（RT-PCR）定量检测 MYH3 和 MYH8 的表达。通过 CT 值进行绝对定量。
结果
在下颌前突患者中，MYH3 上调（8.5，P<0.001），在下颌后缩患者中，MYH3 上调（2.8，P<0.043）。仅在下颌后缩患者中，MYH8 上调（4.0，P<0.063），而在下颌前突患者中下调（-1.6）。下颌后缩患者术前和术后 MYH3 值相关（P<0.005）。
结论
MYH3 和 MYH8 可能在正颌手术后和口腔颌面矫形中发挥作用。
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