Research Institute for Innovation in Sustainable Chemistry, National Institute of Advanced Industrial Science and Technology (AIST), Tsukuba Central 5-2, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8565, Japan.
J Biosci Bioeng. 2010 Dec;110(6):690-5. doi: 10.1016/j.jbiosc.2010.07.003. Epub 2010 Jul 31.
The aim of this research was the application of a two-stage electrodialysis (ED) method for glyceric acid (GA) recovery from fermentation broth. First, by desalting ED, glycerate solutions (counterpart is Na+) were concentrated using ion-exchange membranes, and the glycerate recovery and energy consumption became more efficient with increasing the initial glycerate concentration (30 to 130 g/l). Second, by water-splitting ED, the concentrated glycerate was electroconverted to GA using bipolar membranes. Using a culture broth of Acetobacter tropicalis containing 68.6 g/l of D-glycerate, a final D-GA concentration of 116 g/l was obtained following the two-stage ED process. The total energy consumption for the D-glycerate concentration and its electroconversion to D-GA was approximately 0.92 kWh per 1 kg of D-GA.
本研究的目的是应用两段式电渗析（ED）法从发酵液中回收甘油酸（GA）。首先，通过脱盐 ED，使用离子交换膜将甘油酸盐溶液（对应物为 Na+）浓缩，并且随着初始甘油酸盐浓度（30 至 130 g/l）的增加，甘油酸盐的回收率和能耗变得更加高效。其次，通过水分解 ED，使用双极膜将浓缩的甘油酸盐电转化为 GA。使用含有 68.6 g/l D-甘油酸的热带醋杆菌培养液，通过两段式 ED 工艺，最终获得 116 g/l 的 D-GA 浓度。D-甘油酸的浓缩及其电转化为 D-GA 的总能耗约为每 1 kg D-GA 0.92 kWh。
