[运用多重荧光原位杂交技术检测多发性骨髓瘤患者的复杂染色体畸变]

[Detection of complex chromosomal aberrations in patients with multiple myeloma using multiplex fluorescence in situ hybridization].

作者信息

Jiang Yuan-qiang, Chen Li-juan, Zhu Yu, Qiu Hai-rong, Wang Rong, Xu Jia-ren, Lu Hua, Li Jian-yong

机构信息

Department of Hematology, the Wuxi First Affiliated Hospital, Nanjing Medical University, Wuxi, Jiangsu, 214002 PR China.

出版信息

Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2010 Aug;27(4):441-4. doi: 10.3760/cma.j.issn.1003-9406.2010.04.018.

DOI:10.3760/cma.j.issn.1003-9406.2010.04.018

PMID:20677154

Abstract

OBJECTIVE

To explore the value of multiplex fluorescence in situ hybridization (M-FISH) in the detection of the complex chromosomal aberrations (CCAs) in multiple myeloma (MM).

METHODS

M-FISH was used in 10 MM patients with CCAs detected by conventional cytogenetics (CC) using R-banding to refine the rearrangement of CCAs and identify the characteristics of marker chromosome.

RESULTS

M-FISH confirmed the 29 structural aberrations shown by CC analysis, and also confirmed the specific source of 21 types of chromosomal aberration, which were not detected by CC analysis. Among them, t(2;15)(q33;q22), t(6;7)(q23;q34), t(8;11) (q24;q23), t(1;14)(q10;q32) and t(X;1)(q26;q25) were new chromosomal aberrations. The median survival time of 9 MM patients with CCAs was 23 months and evidently shorter than that of MM patients without CCAs, with the mean survival time being 34 months.

CONCLUSION

M-FISH could refine CCAs in MM patients, find or correct the missed or misidentified abnormalities analyzed by CC. It has provided one of the essential methods for the research of chromosomal aberrations in MM.

摘要

目的

探讨多重荧光原位杂交（M-FISH）技术在检测多发性骨髓瘤（MM）复杂染色体畸变（CCA）中的价值。

方法

应用M-FISH技术对10例经常规细胞遗传学（CC）采用R显带检测出CCA的MM患者进行检测，以细化CCA的重排并鉴定标记染色体的特征。

结果

M-FISH证实了CC分析显示的29种结构畸变，还证实了CC分析未检测到的21种染色体畸变的具体来源。其中，t(2;15)(q33;q22)、t(6;7)(q23;q34)、t(8;11)(q24;q23)、t(1;14)(q10;q32)和t(X;1)(q26;q25)为新的染色体畸变。9例有CCA的MM患者的中位生存时间为23个月，明显短于无CCA的MM患者，后者平均生存时间为34个月。