Key Laboratory of Analytical Chemistry for Life Science of Shaanxi Province, Engineering Research Center of Historical and Cultural Heritage Protection, Ministry of Education, School of Chemistry and Materials Science, Shaanxi Normal University, Xi'an 710062, PR China.
Biosens Bioelectron. 2010 Oct 15;26(2):643-8. doi: 10.1016/j.bios.2010.07.003. Epub 2010 Jul 8.
Poly(luminol-aniline) nanowires composite (PLANC) was synthesized on the surface of graphite electrode by electro-oxidizing the mixture of luminol with aniline in the H2SO4 acidic medium. The properties of the nanowires composite were characterized by transmission electron microscopy, electrogenerated chemiluminescence and electrochemical impedance spectroscopy. The investigating results showed that: firstly, while the luminol alone was electro-oxidized on the surface of the graphite electrode with the proposed synthetic method, only the polyluminol film was formed on electrode surface. However, while a suitable amount of the aniline was present in the luminol solution, the PLANC could be formed on the electrode surface and this PLANC presented better ECL properties for H2O2 compared with pure polyluminol film; secondly, since the PLANC modified electrode not only provided a larger surface area for higher glucose enzyme loading but also formed the nano-structured interface on the electrode surface to improve the analytical performances of the electrogenerated chemiluminescence (ECL) biosensor for glucose, the resulting ECL biosensor presented good response to glucose and a novel ECL biosensor for glucose was proposed.
聚(鲁米诺-苯胺)纳米线复合材料(PLANC)通过在 H2SO4 酸性介质中电氧化鲁米诺与苯胺的混合物,在石墨电极表面合成。纳米线复合材料的性质通过透射电子显微镜、电致化学发光和电化学阻抗谱进行了表征。研究结果表明:首先,当单独用电化学氧化法在石墨电极表面氧化鲁米诺时,仅在电极表面形成聚鲁米诺膜。然而,当在鲁米诺溶液中存在适量的苯胺时,可在电极表面形成 PLANC,与纯聚鲁米诺膜相比,该 PLANC 对 H2O2 具有更好的 ECL 性能;其次,由于 PLANC 修饰电极不仅提供了更大的表面积以增加葡萄糖酶的负载量,而且在电极表面形成了纳米结构的界面,从而提高了电致化学发光(ECL)生物传感器对葡萄糖的分析性能,所得到的 ECL 生物传感器对葡萄糖有良好的响应,提出了一种用于葡萄糖的新型 ECL 生物传感器。