Ministry of Education Key Laboratory of Analysis and Detection for Food Safety, Department of Chemistry, Fuzhou University, Fuzhou, Fujian, China.
Biosens Bioelectron. 2010 Feb 15;25(6):1414-9. doi: 10.1016/j.bios.2009.10.042. Epub 2009 Nov 5.
A new biocompatible ECL biosensor based on enzyme/titanate nanotubes/chitosan composite film was developed for the determination of analytes in biological samples. In the fabrication of the new ECL biosensor, biocompatible titanate nanotubes (TNTs) and a model enzyme, i.e., choline oxidase (ChOX), were immobilized on a chitosan modified glassy carbon electrode (GCE) via electrostatic adsorption and covalent interaction, respectively. By this ECL biosensor, choline was enzymatically oxidized to hydrogen peroxide and detected by a sensitive luminol ECL system. The use of TNTs not only provided a biocompatible microenvironment for the immobilized enzyme, which resulted in an excellent stability and long lifetime of the ECL biosensor, but also exhibited great enhancement towards luminol ECL and thus led to a significant improvement in sensitivity of ECL biosensor. Satisfactory results were obtained when employing this biosensor in assaying the total choline in milk samples. The work would provide a common platform to develop various sensitive, selective and biocompatible ECL biosensors based on using enzyme/TNTs/CHIT composite films.
基于酶/钛酸盐纳米管/壳聚糖复合膜的新型生物相容性 ECL 生物传感器被开发用于测定生物样品中的分析物。在新型 ECL 生物传感器的制备中，通过静电吸附和共价相互作用，将生物相容性的钛酸盐纳米管（TNTs）和模型酶，即胆碱氧化酶（ChOX）固定在壳聚糖修饰的玻碳电极（GCE）上。通过该 ECL 生物传感器，胆碱被酶促氧化为过氧化氢，并通过灵敏的鲁米诺 ECL 系统进行检测。TNTs 的使用不仅为固定化酶提供了生物相容性的微环境，从而导致 ECL 生物传感器具有出色的稳定性和长寿命，而且对鲁米诺 ECL 表现出极大的增强作用，从而显著提高了 ECL 生物传感器的灵敏度。当将该生物传感器用于测定牛奶样品中的总胆碱时，获得了令人满意的结果。这项工作将为基于使用酶/TNTs/CHIT 复合膜开发各种灵敏、选择性和生物相容性的 ECL 生物传感器提供一个通用平台。
