Programa de Pós, Graduação em Bioquímica Toxicológica, Laboratório de Toxicologia de Pesticidas em Peixes, Universidade Federal de Santa Maria, Santa Maria, RS, 97105.900, Brasil.
Arch Environ Contam Toxicol. 2011 May;60(4):665-71. doi: 10.1007/s00244-010-9574-6. Epub 2010 Aug 1.
Antioxidant enzymes and oxidative stress indicators were evaluated in fish exposed to different concentrations of the herbicide Roundup 48% (Monsanto, St. Louis, MO): control (none), 0.45, or 0.95 mg/l. After exposure for 8 days to herbicide, fish were transferred to clean water for a recovery response period (also 8 days). Herbicide increased thiobarbituric acid reactive species in liver and muscle at the higher concentration and in the brain at both concentrations. Protein carbonyl in liver increased after exposure. Catalase (CAT) and superoxide dismutase (SOD) activities and ascorbic acid levels in liver did not change in fish exposed to both concentrations. Glutathione S-transferase (GST) levels decreased at both concentrations. The nonprotein thiol levels decreased at the 0.95 mg/l concentration. During the recovery period, some of the parameters that had altered, such as protein carbonyl content, later recovered. However, some enzymes reacted during this period, e.g., GST increased its activity, possibly indicating a compensatory response against the toxic conditions. In contrast, CAT and SOD activities decreased during the recovery period, indicating herbicide toxicity. Oxidative stress that occurred during the exposure period was likely due to the increased lipid peroxidation and protein carbonyl content. The results concerning oxidative and antioxidant profiles indicate that short-term exposure to herbicide is capable of causing oxidative stress in fish tissues.
抗氧化酶和氧化应激指标在暴露于不同浓度的除草剂 Roundup 48%（孟山都，圣路易斯，密苏里州）的鱼类中进行了评估：对照（无），0.45 或 0.95mg/L。在接触除草剂 8 天后，鱼类被转移到清洁水中进行恢复反应期（也是 8 天）。在较高浓度和两种浓度下，除草剂均增加了肝脏和肌肉中的硫代巴比妥酸反应性物质和大脑中的蛋白质羰基。暴露后肝脏中的蛋白质羰基增加。暴露于两种浓度下，肝脏中的过氧化氢酶（CAT）和超氧化物歧化酶（SOD）活性和抗坏血酸水平没有变化。谷胱甘肽 S-转移酶（GST）水平在两种浓度下均降低。非蛋白巯基水平在 0.95mg/L 浓度下降低。在恢复期间，一些发生改变的参数，如蛋白质羰基含量，后来恢复了。然而，在此期间一些酶发生了反应，例如 GST 增加了其活性，可能表明对有毒条件的补偿反应。相比之下，CAT 和 SOD 活性在恢复期间下降，表明除草剂毒性。暴露期间发生的氧化应激可能是由于脂质过氧化和蛋白质羰基含量增加所致。有关氧化和抗氧化特征的结果表明，短期接触除草剂能够导致鱼类组织中的氧化应激。
