Laboratório de Bioquímica toxicológica e Adaptativa de Peixes, Programa de Pós-graduação em Ciências Biológicas, Bioquímica Toxicológica, Universidade Federal de Santa Maria, Rio Grande do Sul, Brazil.
J Appl Toxicol. 2010 Aug;30(6):590-5. doi: 10.1002/jat.1530.
Cyprinus carpio were exposed under field conditions to 20.87 microg l(-1) of commercial herbicide bispyribac-sodium (Nominee, SC), during 7, 21 and 72 days. Enzymatic parameters such as catalase (CAT), glutathione S-transferase (GST) and acetylcholinesterase (AChE) activities, as well as thiobarbituric acid-reactive substances (TBARS) and protein carbonyl contents were studied in different tissues. After 7 days of exposure, GST activity decreased. At the same period, brain AChE activity increased, but a reduction of activity was observed in muscle tissue. Brain TBARS levels increased at 7 days. After 21 days of exposure liver CAT levels and muscle AChE activities decreased. In the same period, liver protein carbonyl and muscle TBARS increased. After 72 days of exposure in the field, AChE activity was reduced in both brain and muscle. Protein carbonyl contents in liver and brain TBARS levels increased. Muscle AChE activity, TBARS and protein carbonyl can be used as biomarkers of exposure to the herbicide bispyribac-sodium. This study demonstrates effects of exposure to bispyribac-sodium under rice field conditions on oxidative stress parameters in tissues of Cyprinus carpio.
鲤鱼在田间条件下暴露于 20.87μg/L 的商业除草剂双丙戊酸钠（候选物，SC）中，暴露时间分别为 7、21 和 72 天。研究了不同组织中的酶学参数，如过氧化氢酶（CAT）、谷胱甘肽 S-转移酶（GST）和乙酰胆碱酯酶（AChE）活性，以及丙二醛（TBARS）和蛋白质羰基含量。暴露 7 天后，GST 活性降低。在同一时期，大脑 AChE 活性增加，但肌肉组织的活性降低。大脑 TBARS 水平在 7 天时增加。暴露 21 天后，肝 CAT 水平和肌肉 AChE 活性降低。在同一时期，肝蛋白羰基和肌肉 TBARS 增加。暴露 72 天后，大脑和肌肉中的 AChE 活性均降低。肝和脑 TBARS 水平升高，蛋白羰基含量增加。本研究表明，在稻田条件下暴露于双丙戊酸钠会对鲤鱼组织中的氧化应激参数产生影响。
