Liu Qiang, Xiao Yongsheng, He Lin
Department of Pathology & Lab Medicine, University of North Carolina at Chapel Hill, Chapel Hill, NC, USA.
Methods Mol Biol. 2010;656:243-52. doi: 10.1007/978-1-60761-746-4_14.
Surface-assisted laser desorption/ionization mass spectrometry (SALDI-MS) uses inorganic particles or porous surfaces as the energy-mediating means to promote desorption and ionization of low-mass analytes of interest. With good stability during laser ablation, SALDI substrates exhibit reduced background in the low-mass region that is often crowded in conventional matrix-assisted laser desorption/ionization (MALDI) due to matrix fragmentation; a benefit renders SALDI-MS attractive in imaging low-mass species. Practical application of SALDI-MS, however, is hindered by its unsatisfied detection sensitivity for most compounds. With aims of improving MS imaging resolution and sensitivity of low-mass species, we describe an experimental protocol using a hybrid ionization method, termed matrix-enhanced SALDI (ME-SALDI), to detect crucial low-mass species with their spatial distribution in mouse brain tissue.
表面辅助激光解吸/电离质谱（SALDI-MS）使用无机颗粒或多孔表面作为能量介导手段，以促进目标低质量分析物的解吸和电离。由于在激光烧蚀过程中具有良好的稳定性，SALDI基质在低质量区域显示出较低的背景，而在传统的基质辅助激光解吸/电离（MALDI）中，由于基质碎片化，该区域常常很拥挤；这一优势使得SALDI-MS在低质量物种成像方面具有吸引力。然而，SALDI-MS的实际应用受到其对大多数化合物检测灵敏度不足的阻碍。为了提高低质量物种的质谱成像分辨率和灵敏度，我们描述了一种实验方案，使用一种称为基质增强SALDI（ME-SALDI）的混合电离方法，来检测小鼠脑组织中关键低质量物种及其空间分布。
