Tham K T, Cousar J B
Department of Pathology, Veterans Administration Medical Center, Nashville, Tennessee.
J Clin Pathol. 1993 Aug;46(8):766-8. doi: 10.1136/jcp.46.8.766.
During a study of nucleolar organiser regions, a modified silver stain was found to be a sensitive marker for the iron in ringed sideroblasts, more so than Perls's stain when the marrow iron stores were low. To enhance the usefulness of the silver stain, a combined silver Perls method was developed. This stains the ringed sideroblast iron black and haemosiderin blue, thus rendering the detection of ringed sideroblasts easier even when marrow iron stores are excessive. AT the same time, it allows marrow iron content to be evaluated. The silver reagent in this combined method probably shows phosphate rather than the iron present in the abnormal mitochondria in ringed sideroblasts. This facilitates the differential staining of ringed sideroblast "iron" and haemosiderin.
在一项关于核仁组织区的研究中，发现一种改良的银染色法是环形铁粒幼红细胞中铁的敏感标记物，当骨髓铁储存量较低时，其比普鲁士蓝染色更敏感。为提高银染色法的实用性，开发了一种银-普鲁士蓝联合方法。该方法将环形铁粒幼红细胞中的铁染成黑色，含铁血黄素染成蓝色，因此即使在骨髓铁储存过量时，也能更轻松地检测到环形铁粒幼红细胞。同时，它还能评估骨髓铁含量。这种联合方法中的银试剂可能显示的是磷酸盐，而非环形铁粒幼红细胞异常线粒体中的铁。这有助于对环形铁粒幼红细胞的“铁”和含铁血黄素进行鉴别染色。
