Trutko S M, Golovchenko N P, Akimenko V K
Mikrobiologiia. 1978 Jan-Feb;47(1):5-10.
Changes in the electron transport chain of E. coli K-12 were studied as a function of the growth phase and the nature of a terminal electron acceptor in the growth medium. The content of flavins in the preparations of bacterial membranes hardly changed in all cases. The highest concentration of quinones was observed in the bacterial membranes at the stationary growth phase under anaerobic conditions of growth in the presence of nitrate. These membranes contained also the greatest amount of cytochrome beta1. The concentration of cytochrome alpha2 in all the membranes was low and varied among different preparations. All the membranes contained a CO-binding pigment whose content was maximal in the membranes of "nitrate" cells. The membranes of cells grown under aerobic conditions oxidized malate, apart from NADH and lactate, whereas the membranes of cells cultivated under anaerobic conditions in the presence of nitrate oxidized formiate. In most cases, the oxidase activity of the membranes of cells collected at the stationary growth phase was higher cf. the exponential phase.
研究了大肠杆菌K-12电子传递链的变化与生长阶段以及生长培养基中末端电子受体性质的关系。在所有情况下，细菌膜制剂中黄素的含量几乎没有变化。在硝酸盐存在下厌氧生长的稳定生长期，细菌膜中观察到醌的浓度最高。这些膜中还含有最多的细胞色素β1。所有膜中细胞色素α2的浓度都很低，且不同制剂之间有所不同。所有膜都含有一种CO结合色素，其含量在“硝酸盐”细胞的膜中最高。有氧条件下生长的细胞的膜除了能氧化NADH和乳酸外，还能氧化苹果酸，而在硝酸盐存在下厌氧培养的细胞的膜能氧化甲酸。在大多数情况下，稳定生长期收集的细胞的膜的氧化酶活性高于指数生长期。
