Jurkowska Renata Z, Jeltsch Albert
Biochemistry Lab, School of Engineering and Science, Jacobs University Bremen, Bremen, Germany.
Methods Mol Biol. 2010;649:149-61. doi: 10.1007/978-1-60761-753-2_9.
Targeted DNA methylation is a novel and attractive approach for stable silencing of gene expression by epigenetic mechanisms. The potential applications of this concept include cancer treatment, treatment of viral infections and, in general, treatment of any disease that could be attenuated by the stable repression of known target genes. We review the literature on targeted DNA methylation and gene silencing, summarize the achievements and the challenges that remain, and discuss technical issues critical for this approach.
靶向DNA甲基化是一种通过表观遗传机制稳定沉默基因表达的新颖且有吸引力的方法。这一概念的潜在应用包括癌症治疗、病毒感染治疗，以及一般而言，任何可通过稳定抑制已知靶基因而减轻症状的疾病的治疗。我们回顾了关于靶向DNA甲基化和基因沉默的文献，总结了已取得的成就和仍存在的挑战，并讨论了该方法的关键技术问题。
