Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources (Ministry of Education of China), College of Chemistry and Chemical Engineering, Guangxi Normal University, Guilin, P. R. China.
J Sep Sci. 2010 Aug;33(15):2356-60. doi: 10.1002/jssc.201000140.
A novel method for the determination of galanthamine (GAL) in Bulbus Lycoridis Radiatae has been developed based on coupling CE with an end-column tris(2,2'-bipyridyl)ruthenium(II) electrochemiluminescence (ECL). Parameters affecting CE separation and ECL detection were investigated and optimized. Baseline separation of GAL from other components in the Bulbus Lycoridis Radiatae sample was achieved with an 18 mmol/L phosphate running buffer at pH 9.0. Under the optimized conditions: 12 kV CE-separation voltage, ECL detection potential at 1.25 V with 5 mmol/L Ru(bpy)(3)(2+) and 50 mmol/L phosphate buffer at pH 7.5 in the detection reservoir, the linear range of GAL concentration was from 0.8 ng/mL to 2 microg/mL, whereas the detection limit was 0.25 ng/mL (S/N=3). The proposed method was successfully demonstrated for the determination of GAL in Bulbus Lycoridis Radiatae.
建立了一种基于 CE 与末端柱三(2,2'-联吡啶)钌(II)电化学发光(ECL)偶联测定石蒜中加兰他敏(GAL)的新方法。考察并优化了影响 CE 分离和 ECL 检测的参数。在 pH 9.0 的 18 mmol/L 磷酸盐运行缓冲液中，实现了 GAL 与石蒜样品中其他成分的基线分离。在优化条件下：12 kV CE 分离电压，ECL 检测电位为 1.25 V，检测池中含有 5 mmol/L Ru(bpy)(3)(2+)和 50 mmol/L 磷酸盐缓冲液，GAL 浓度的线性范围为 0.8 ng/mL 至 2 microg/mL，检测限为 0.25 ng/mL(S/N=3)。该方法成功地用于测定石蒜中的 GAL。
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