五味子醇甲对β-淀粉样蛋白损伤的PC12细胞的保护及治疗作用
[Protective and therapeutic effects of schisandrol A on Abeta damaged PC12 cells].
作者信息
Xiao Fei, Weng Wen, Luo Huan-Min
机构信息
Jinan University, School of Medicine, Guangzhou 510632, China.
出版信息
Zhong Yao Cai. 2010 Mar;33(3):397-401.
OBJECTIVES
To observe the protective and therapeutic effect of schisandrol A on the Abeta damaged PC12 cells.
METHODS
PC12 cells were damaged by Abeta in vitro. Morphological changes were observed and the number of cells with neurite was analyzed by phase contrast microscope. The cell viability of PC12 cells was determined by the MTT method.
RESULTS
Many dispirited cells with atrophied or fragmented neurites in the Abeta damaged PC12 cells were observed under the microscope. More vital cells with longer neurites were observed in the schisandrol A treated PC12 cells and the number of cells with neurite increased. The difference of cell viability between the two groups was statistical significant.
CONCLUSION
Schisandrol A can antagonize the neurotoxicity of Abeta and has protective and therapeutic efficacy on Abeta damaged PC12 cells.
目的
观察五味子醇甲对β-淀粉样蛋白（Aβ）损伤的PC12细胞的保护及治疗作用。
方法
体外培养PC12细胞，用Aβ对其进行损伤。通过相差显微镜观察细胞形态变化并分析有神经突的细胞数量。采用MTT法测定PC12细胞的活力。
结果
显微镜下观察发现，Aβ损伤的PC12细胞中许多细胞萎靡不振，神经突萎缩或断裂。在五味子醇甲处理的PC12细胞中观察到更多具有更长神经突的活力细胞，且有神经突的细胞数量增加。两组细胞活力差异具有统计学意义。
结论
五味子醇甲可拮抗Aβ的神经毒性，对Aβ损伤的PC12细胞具有保护及治疗作用。
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