铅和汞对儿童血液蛋白质组的影响。
Effects of lead and mercury on the blood proteome of children.
机构信息
Department of Chemistry, SUNY Oswego, Oswego, New York 13126, USA.
出版信息
J Proteome Res. 2010 Sep 3;9(9):4443-53. doi: 10.1021/pr100204g.
Heavy metal exposure in children has been associated with a variety of physiological and neurological problems. The goal of this study was to utilize proteomics to enhance the understanding of biochemical interactions responsible for the health problems related to lead and mercury exposure at concentrations well below CDC guidelines. Blood plasma and serum samples from 34 children were depleted of their most abundant proteins using antibody-based affinity columns and analyzed using two different methods, LC-MS/MS and 2-D electrophoresis coupled with MALDI-TOF/MS and tandem mass spectrometry. Apolipoprotein E demonstrated an inverse significant association with lead concentrations (average being one microgram/deciliter) as deduced from LC-MS/MS and 2-D electrophoresis and confirmed by Western blot analysis. This coincides with prior findings that Apolipoprotein E genotype moderates neurobehavioral effects in individuals exposed to lead. Fifteen other proteins were identified by LC-MS/MS as proteins of interest exhibiting expressional differences in the presence of environmental lead and mercury.
儿童重金属暴露与多种生理和神经问题有关。本研究的目的是利用蛋白质组学来增强对生物化学相互作用的理解，这些相互作用负责解释浓度远低于疾病预防控制中心指导方针的铅和汞暴露相关的健康问题。使用基于抗体的亲和柱从 34 名儿童的血浆和血清样本中去除其最丰富的蛋白质，并使用两种不同的方法进行分析，即 LC-MS/MS 和 2-D 电泳与 MALDI-TOF/MS 和串联质谱。载脂蛋白 E 表现出与铅浓度呈显著负相关（平均为每微克/分升），这是从 LC-MS/MS 和 2-D 电泳推断出来的，并通过 Western blot 分析得到证实。这与先前的发现一致，即载脂蛋白 E 基因型调节个体暴露于铅时的神经行为影响。通过 LC-MS/MS 鉴定出另外 15 种蛋白质作为感兴趣的蛋白质，这些蛋白质在存在环境铅和汞的情况下表现出表达差异。
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