Department of Molecular Apidology, Institute of Molecular Biology, Slovak Academy of Sciences, Bratislava, Slovakia.
J Agric Food Chem. 2010 Aug 11;58(15):8776-81. doi: 10.1021/jf101583s.
The 55 kDa major protein of royal jelly, named apalbumin 1, is an authentic protein of honey and pollen pellet, and for its quantification an enzyme-linked immunosorbent assay (ELISA) was developed using specific polyclonal anti-apalbumin 1 antibody. The limit of detection for apalbumin 1 was 2 ng mL(-1). The floral honeys contained apalbumin 1 as follows: acacia, 0.011%; linden, 0.010%; chestnut, 0.029%; rape, 0.010%; and dandelion, 0.014%. The saccharose syrup honey contained only 0.001% of apalbumin 1. The average amount of apalbumin 1 relating to the total protein content of analyzed honey samples was 23.39%, whereas in SCCH apalbumin 1 presented only 4.81% of total proteins of honey. Apalbumin 1 is thermostabile in honey at 80 degrees C incubated for 40 min. ELISA results show good precision in the evaluation of apalbumin 1 quantity in honey (CV ranged from 0.69 to 4.25%).
蜂王浆 55kDa 主要蛋白质，命名为白蛋白 1，是蜂蜜和花粉粒的一种真正蛋白质，针对其定量分析，我们开发了一种酶联免疫吸附测定法（ELISA），使用特异性多克隆抗白蛋白 1 抗体。白蛋白 1 的检测限为 2ng/mL。花蜜中含有白蛋白 1，具体含量如下：刺槐，0.011%；椴树，0.010%；栗树，0.029%；油菜，0.010%；蒲公英，0.014%。蔗糖糖浆蜂蜜仅含有 0.001%的白蛋白 1。分析的蜂蜜样本中，白蛋白 1 与总蛋白含量的平均比例为 23.39%，而在 SCCH 中，白蛋白 1 仅占蜂蜜总蛋白的 4.81%。在 80°C 下孵育 40 分钟，白蛋白 1 在蜂蜜中具有热稳定性。ELISA 结果表明，该方法在评估蜂蜜中白蛋白 1 含量方面具有良好的精密度（CV 范围为 0.69%至 4.25%）。
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