Yamaguchi Kikuji, He Shaoyu, Li Zhengyue, Murata Kiyoshi, Hitomi Nobuyuki, Mozumi Manaho, Ariga Risa, Enomoto Toshiki
College of Plant Protection, Yunnan Agricultural University, Yunnan, China.
Biosci Biotechnol Biochem. 2013;77(6):1310-2. doi: 10.1271/bbb.130013. Epub 2013 Jun 7.
Rabbit polyclonal antibody produced by a major royal jelly protein 1 (MRJP1) specific peptide reacted only with a MRJP1. Indirect ELISA with the antibody revealed a MRJP1 level of 4.12-4.67 g/100 g in different company's royal jelly, which almost agreed with that of a hexametric form of MRJP1 (apisin) measured by high performance liquid chromatography. These results suggest that MRJP1 exists mainly as apisin in royal jelly.
由主要蜂王浆蛋白1（MRJP1）特异性肽产生的兔多克隆抗体仅与MRJP1发生反应。用该抗体进行的间接酶联免疫吸附测定显示，不同公司蜂王浆中MRJP1的水平为4.12 - 4.67克/100克，这与通过高效液相色谱法测得的六聚体形式的MRJP1（apisin）的水平几乎一致。这些结果表明，MRJP1在蜂王浆中主要以apisin的形式存在。
