通过间接酶联免疫吸附测定法对新鲜蜂王浆中主要蜂王浆蛋白1进行定量分析。
Quantification of major royal jelly protein 1 in fresh royal jelly by indirect enzyme-linked immunosorbent assay.
作者信息
Yamaguchi Kikuji, He Shaoyu, Li Zhengyue, Murata Kiyoshi, Hitomi Nobuyuki, Mozumi Manaho, Ariga Risa, Enomoto Toshiki
机构信息
College of Plant Protection, Yunnan Agricultural University, Yunnan, China.
出版信息
Biosci Biotechnol Biochem. 2013;77(6):1310-2. doi: 10.1271/bbb.130013. Epub 2013 Jun 7.
Rabbit polyclonal antibody produced by a major royal jelly protein 1 (MRJP1) specific peptide reacted only with a MRJP1. Indirect ELISA with the antibody revealed a MRJP1 level of 4.12-4.67 g/100 g in different company's royal jelly, which almost agreed with that of a hexametric form of MRJP1 (apisin) measured by high performance liquid chromatography. These results suggest that MRJP1 exists mainly as apisin in royal jelly.
由主要蜂王浆蛋白1（MRJP1）特异性肽产生的兔多克隆抗体仅与MRJP1发生反应。用该抗体进行的间接酶联免疫吸附测定显示，不同公司蜂王浆中MRJP1的水平为4.12 - 4.67克/100克，这与通过高效液相色谱法测得的六聚体形式的MRJP1（apisin）的水平几乎一致。这些结果表明，MRJP1在蜂王浆中主要以apisin的形式存在。
Zotero 插件