ESTM, Polytechnic Institute of Leiria, Peniche, Portugal.
J Agric Food Chem. 2010 Aug 11;58(15):8854-61. doi: 10.1021/jf101646y.
In the present study, water, ethanolic, and methanolic extracts from seven selected wild fruits originally from the Mediterranean area, namely, strawberry tree ( Arbutus unedo L., AU), azarole ( Crataegus azarolus L., CA), common hawthorn ( Crataegus monogyna L., CM), blackthorn ( Prunus spinosa L., PS), dog rose ( Rosa canina L., RC), elm-leaf blackberry ( Rubus ulmifolius Schott, RU), and rowan ( Sorbus aucuparia L., SA), were analyzed for the total amount and profile of phenolic compounds and for the in vitro antioxidant activity against the DPPH and ABTS radicals (study 1). The seven fruits showed different chemical compositions, which consequently led to different antioxidant potentials. Among the seven fruits initially analyzed, AU, CM, RC, and RU had the highest amount of phenolic compounds and displayed the greatest antioxidant activity in vitro. Extracts from these four fruits were tested as inhibitors of lipid oxidation in raw pork burger patties subjected to refrigerated storage at 2 degrees C for 12 days (study 2). The quantitative measurements of thiobarbituric acid reactive substances (TBA-RS), hexanal content, and color stability were used as indicators of oxidative reactions. The four selected fruits displayed intense antioxidant activity against lipid oxidation, which highlights the potential usage of these fruits as ingredients for the manufacture of healthy meat products. Among them, RC and AU were particularly efficient as their protective effect against lipid oxidation was more intense than that displayed by quercetin (230 mg/kg of burger patty).
在本研究中,我们分析了七种源自地中海地区的野生水果(包括草莓树、酸樱桃、普通山楂、黑刺李、狗蔷薇、欧洲刺李和花楸)的水提物、醇提物和水提醇沉物,以评估其总酚含量和组成,并测定其对 DPPH 和 ABTS 自由基的体外抗氧化活性(研究 1)。这七种水果的化学成分存在差异,这导致了它们的抗氧化潜力不同。在最初分析的七种水果中,草莓树、普通山楂、狗蔷薇和欧洲刺李的总酚含量最高,体外抗氧化活性最强。在研究 2 中,我们将这四种水果的提取物作为抑制剂,用于抑制在 2°C 下冷藏 12 天的生猪肉汉堡肉饼中的脂质氧化。我们使用硫代巴比妥酸反应物(TBA-RS)、己醛含量和颜色稳定性的定量测量来评估氧化反应。这四种选定的水果显示出强烈的抗氧化活性,可以抑制脂质氧化,这突出了这些水果作为制造健康肉类产品的成分的潜在用途。其中,狗蔷薇和草莓树的抗氧化效果尤其显著,其对脂质氧化的保护作用强于槲皮素(230mg/kg 汉堡肉饼)。